ANALYSIS OF EXOSOMES FROM CISPLATIN RESISTANT AND SENSITIVE OVARIAN CANCER

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。Howell博士的研究小组通过微阵列分析发现，独特的外泌体标志物在顺铂（DDP）耐药细胞中过度表达。纯化外泌体的生化分析表明，耐药细胞释放的外泌体显著富含DDP的推定外排转运体。来自DDP耐药细胞的外泌体也积累了更高水平的DDP，这增加了它们可能有助于从耐药细胞输出药物的可能性（耐药细胞有积累缺陷）。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Dr. Howell's group has found by microarray analysis that unique exosomal markers are over-expressed in cisplatin (DDP) resistant cells. Biochemical analysis of purified exosomes showed that resistant cells release exosomes that are significantly enriched in putative efflux transporters of DDP. The exosomes from DDP resistant cells also accumulate higher levels of DDP, raising the possibility that they maight be instrumental in the export of the drug from resistant cells (resistant cells are accumulation defective.)