PROPERTIES OF SINGLE ASSEMBLIES OF LIGHT HARVESTER LH2

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。我们正在研究LH2复合体和可从其中可逆提取的UP-二肽的单一组装中的光漂白和转移动力学。每个LH2含有18个细菌叶绿素(B850)和9个捕收剂(B800)。研究了不同条件下的荧光寿命、偏振、相关时间和漂白动力学。主要目标包括测量长时间尺度的蛋白质动力学。以前的极化实验已经揭示了与单体LH2组装中的激子迁移相关的构象动力学。此外，最近还探测和研究了来自较高激发态的蓝光发射。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are examining the photobleaching and transfer dynamics in single assemblies of the LH2 complexes and the up-dipeptides that can be reversibly extracted from them. Each LH2 contains eighteen bacteriochlorophylls (B850) and nine collectors (B800). The fluorescence lifetimes, polarization, correlation times and bleaching kinetics are being studied under a variety of conditions. The primary goals include measurements of long time scale protein dynamics. Previous polarization experiments have revealed conformational dynamics associated with excitonic migration in the monomeric LH2 assembly. Furthermore, blue emission originating from higher excited states was recently detected and investigated.