RAMAN FLOW CYTOMETRY FOR DRUG DISCOVERY AND DIAGNOSTICS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。对生物系统进行定量、高通量分子测量的能力是生物医学研究的许多领域的关键需求。该生物工程研究伙伴关系（BRP）旨在开发一个强大的新的分析平台，用于基于拉曼流式细胞术的高通量筛选和选择。该合作伙伴关系将开发新的分析仪器，用于化学合成和筛选的光学编码聚合物树脂，以及具有独特光学特性的纳米结构材料，用于敏感的报告和编码。这项新技术将对流动中的单个颗粒进行拉曼光谱分析，从而在敏感的多重检测、药物发现和诊断方面实现新的应用。拉曼流式细胞仪仪器和应用将由一个合作伙伴关系开发，该合作伙伴关系包括来自学术界、政府和工业界的工程师、生物学家和化学家。在合作的第一年，我们将修改一个商业粒子分选机，以检测单个粒子的单个拉曼振动带，并根据它们的光学特征对这些粒子进行分选。在第2-5年，我们将发展收集和分析单个粒子的完整拉曼光谱的能力。同时，该合作伙伴关系将开发新的编码和报告策略，用于多重分子分析和分离。这种拉曼流式细胞术技术将应用于细菌病原体及其毒素的治疗和诊断的开发。拉曼流式细胞术将是一种重要的和通用的新的分析和分离能力，将影响许多领域的基础和应用生物医学研究除了这里提出的应用。该合作项目推动核心研发项目2（分子组装）和4（光谱流式细胞术）。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The ability to make quantitative, high throughput molecular measurements of biological systems is a critical need for many areas of biomedical research. This Bioengineering Research Partnership (BRP) aims to develop a powerful new analytical platform for high throughput screening and selection based on Raman Flow Cytometry. This Partnership will develop new analytical instrumentation, optically encoded polymer resins for chemical synthesis and screening, and nanostructured materials with unique optically properties for sensitive reporting and encoding. The new technology will perform Raman spectroscopy on single particles in flow to enable new applications in sensitive multiplexed detection, drug discovery, and diagnostics. The Raman Flow Cytometry instrumentation and applications will be developed by a Partnership involving engineers, biologists, and chemists from academia, government and industry. In the first year of the Partnership, we will modify a commercial particle sorter to detect individual Raman vibrational bands from single particles and sort these particles based on their optical signature. In Years 2-5, we will develop the ability to collect and analyze complete Raman spectra from single particles. In parallel, the partnership will develop new encoding and reporting strategies for multiplexed molecular analysis and separation. This Raman Flow Cytometry technology will be applied to the development of therapeutics and diagnostics for bacterial pathogens and their toxins. Raman Flow Cytometry will be an important and general new analytical and separation capability that will impact many areas of basic and applied biomedical research in addition to the applications proposed here. This collaborative project drives Core R&D Projects 2 (Molecular Assembly) and 4 (Spectral Flow Cytometry).