AMINO ACID COMPOSITION OF COMPOUNDS ISOLATED FROM ENTAMOEBA HISTOLYTICA

从溶组织内阿米巴中分离的化合物的氨基酸组成

• 批准号: 7369028
• 负责人: RAUL N ONDARZA
• 金额: $ 0.21万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7369028
• 关键词: AMINO; ACID; COMPOSITION; COMPOUNDS; ISOLATED

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Molecular characterization of a gene that codifies for a disulfide reductase; the low molecular weight thiol compound and the enzyme that participate in the detoxifying mechanism of Entamoeba histolytica. We are looking for a thiol compound that substitutes in Entamoeba histolytica the absence of glutathione and disulfide reducing enzyme. By HPLC and using the fluorescent reagent monobromobimane we have been able to isolate two thiol compounds that have the characteristics of glutathione-spermidine and trypanothione [N1,N8,bis(L-gama-glutamyl-L-hemicysteinil-glycil)spermidine]. The mass spectrometry analysis will give a solid support to other results on the aminoacid composition obtained after acid hydrolisis of the two compounds isolated and purified from Entamoeba histolytica , as well as the results obtained by coelution with standards donated by the discoverer of these compounds from Crithidia fasciculata.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。编码二硫还原酶的基因的分子特征;低分子量硫醇化合物和参与溶组织内阿米巴解毒机制的酶。我们正在寻找一种巯基化合物，它可以取代溶组织内阿米巴中谷胱甘肽和二硫键还原酶的缺失。通过HPLC和使用荧光试剂monobromobimane，我们已经能够分离出两种具有谷胱甘肽-亚精胺和锥虫硫酮特征的硫醇化合物[N1，N8，双（L-γ-谷氨酰-L-半胱氨酸-甘氨酸）亚精胺]。质谱分析将为从溶组织内阿米巴中分离纯化的两种化合物酸水解后获得的氨基酸组成的其他结果以及与这些化合物的发现者捐赠的标准品共洗脱获得的结果提供坚实的支持来自束状内阿米巴的化合物。