RIBOSOME ASSEMBLY

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。在真核生物中，80种核糖体蛋白和170多种核糖体组装因子必须相互作用，并与rRNA以协调的方式相互作用才能产生功能性核糖体亚基。然而，在大多数情况下，这些蛋白质中的哪一种一起起作用以及它们作用的底物尚未确定。现在重要的是建立这些核糖体蛋白或组装因子与其他蛋白质之间的相互作用。我们已经确定了在组装核糖体中存在的两个亚复合物- Nop7/Ytm1/Erb1亚复合物和Rpf2/Rrs1/rpL5/rpL11/5SrRNA亚复合物。有趣的是，Ytm1、Erb1和Nop7也参与其他细胞过程，包括DNA复制和微管功能。为了更好地理解这些蛋白质在核糖体生物发生和其他途径中的作用，我们使用每个蛋白质或每个蛋白质内的结构域作为诱饵，在系统的双杂交筛选中识别相互作用的蛋白质。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In eukaryotes, 80 ribosomal proteins and more than 170 ribosome assembly factors must interact with each other and with rRNA in a coordinated fashion to produce functional ribosomal subunits. However, in most cases, which of these proteins function together and the substrates upon which they act have not been identified. It is now important to establish interactions between these ribosomal proteins or assembly factors and other proteins. We have identified two subcomplexes present in assembling ribosomes--the Nop7/Ytm1/Erb1 subcomplex and the Rpf2/Rrs1/rpL5/rpL11/5SrRNA subcomplex. Interestingly, Ytm1,Erb1, and Nop7 also have been implicated in other cellular processes, including DNA replication and microtubule function. To better understand the roles of these proteins in ribosome biogenesis and in other pathways, we are using each protein or domains within each protein, as baits in a systematic two-hybrid screen to identify interacting proteins.