RIBOSOME ASSEMBLY

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In eukaryotes, 80 ribosomal proteins and more than 170 ribosome assembly factors must interact with each other and with rRNA in a coordinated fashion to produce functional ribosomal subunits. However, in most cases, which of these proteins function together and the substrates upon which they act have not been identified. It is now important to establish interactions between these ribosomal proteins or assembly factors and other proteins. We have identified two subcomplexes present in assembling ribosomes--the Nop7/Ytm1/Erb1 subcomplex and the Rpf2/Rrs1/rpL5/rpL11/5SrRNA subcomplex. Interestingly, Ytm1,Erb1, and Nop7 also have been implicated in other cellular processes, including DNA replication and microtubule function. To better understand the roles of these proteins in ribosome biogenesis and in other pathways, we are using each protein or domains within each protein, as baits in a systematic two-hybrid screen to identify interacting proteins.