BIOGENESIS OF RIBOSOMES & SPLICEOSOMES IN SACCHAROMYCES CEREVISIAE

核糖体的生物发生

• 批准号: 6221041
• 负责人: JOHN L. WOOLFORD
• 金额: $ 0.13万
• 依托单位: MELLON PITTS CORPORATION (MPC CORP)
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6221041
• 关键词: biomedical resource; cognition; human tissue; nervous system; psychology

The long term objective of this research is to investigate the molecular mechanism for the generation of the diversities of immune receptors. The V, (D), and J segments of the Ig and TCR genes are assembled by somatic recombination during the lymphoid differentiation. These recombination processes are mediated by the recombination signal sequences (Rss) and the V (D)J recombinase. To elucidate the molecular mechanisms of the V (D)J recombination, it is essential to characterize lymphoid-specific proteins that bind the Rss. We have cloned the cDNA for a protein, Rc, which is predominantly expressed in thymocytes, by the ability of Rc to bind the Rss. Subsequently, we have shown the Rc binds the kappa B motif and the Ig kappa chain gene enhancer as well. The aim of this project is to investigate the biological role of Rc in V (D)J recombination. The primary structure of Rc will be predicted from its CDNA and possible structural domains will be defined by comparing with p roteins present in the databases. The information may shed light on how Rc can be involved in V (D)J recombination.

本研究的长期目标是调查 免疫紊乱产生的分子机制 受体。 IG和TCR基因的V、（D）和J区段是 在淋巴细胞增殖过程中通过体细胞重组组装 分化 这些重组过程是由 重组信号序列（Rss）和V（D）J重组酶。 到 阐明了V（D）J重组的分子机制， 对于表征淋巴特异性蛋白质是必不可少的， Rss.我们克隆了一种蛋白质Rc的cDNA，该蛋白质主要是 在胸腺细胞中表达，通过Rc结合Rss的能力。 随后，我们发现Rc与κ B基序和IG结合 κ链基因增强子。 该项目的目的是 研究Rc在V（D）J重组中的生物学作用。 的 根据Rc的cDNA序列预测其一级结构， 结构域将通过与存在的前蛋白比较来定义 在数据库里。 这些信息可能会揭示Rc如何能够 参与V（D）J重组。