FACTORS RPF2 & RRS1 RECRUIT 5S RRNA & RPL5 & RPL11 INTO NASCENT RIBS

因素 RPF2

• 批准号: 7722264
• 负责人: JOHN L. WOOLFORD
• 金额: $ 0.11万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722264
• 关键词: Binding; Cell Nucleolus; Computer Retrieval of Information on Scientific Projects Database; Cytoplasm; DNA Sequence Rearrangement; Eukaryota; Eukaryotic Cell; Funding; Grant; Institution; Neighborhoods; Nucleoplasm; Process; Proteins; RNA, Ribosomal, 5S; Recruitment Activity; Research; Research Personnel; Resources; Ribonucleoproteins; Ribosomal Proteins; Ribosomal RNA; Ribosomes; Source; United States National Institutes of Health; Yeasts; cofactor; in vitro Assay; particle; rRNA Precursor; rib bone structure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. More than 170 proteins are necessary for assembly of ribosomes in eukaryotes. However, cofactors that function with each of these proteins, substrates on which they act, and the precise functions of assembly factors--e.g., recruiting other molecules into preribosomes or triggering structural rearrangements of pre-rRNPs--remain mostly unknown. Here we investigated the recruitment of two ribosomal proteins and 5S ribosomal RNA (rRNA) into nascent ribosomes. We identified a ribonucleoprotein neighborhood in preribosomes that contains two yeast ribosome assembly factors, Rpf2 and Rrs1, two ribosomal proteins, rpL5 and rpL11, and 5S rRNA. Interactions between each of these four proteins have been confirmed by binding assays in vitro. These molecules assemble into 90S preribosomal particles containing 35S rRNA precursor (pre-rRNA). Rpf2 and Rrs1 are required for recruiting rpL5, rpL11, and 5S rRNA into preribosomes. In the absence of association of these molecules with pre-rRNPs, processing of 27SB pre-rRNA is blocked. Consequently, the abortive 66S pre-rRNPs are prematurely released from the nucleolus to the nucleoplasm, and cannot be exported to the cytoplasm.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在真核生物中，超过170种蛋白质是核糖体组装所必需的。然而，与这些蛋白质中的每一种起作用的辅因子、它们作用的底物以及组装因子的精确功能--例如，招募其他分子进入前核糖体或触发前rRNP的结构重排-仍然是未知的。在这里，我们研究了两个核糖体蛋白和5S核糖体RNA（rRNA）到新生核糖体的招聘。我们确定了一个核糖核蛋白附近的preribosomes，包含两个酵母核糖体组装因子，Rpf 2和Rrs 1，两个核糖体蛋白，rpL 5和rpL 11，和5S rRNA。这四种蛋白质之间的相互作用已经通过体外结合试验得到证实。这些分子组装成含有35 S rRNA前体（pre-rRNA）的90 S前核糖体颗粒。Rpf 2和Rrs 1是将rpL 5、rpL 11和5S rRNA募集到前核糖体中所必需的。在不存在这些分子与pre-rRNP的缔合的情况下，27 SB pre-rRNA的加工被阻断。因此，败育的66 S pre-rRNP过早地从核仁释放到核质，并且不能输出到细胞质。