LIGANDS OF MOONLIGHTING RIBOSOME ASSEMBLY FACTORS

兼职核糖体组装因子的配体

• 批准号: 7420671
• 负责人: JOHN L. WOOLFORD
• 金额: $ 0.5万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-20 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7420671
• 关键词: library; proteins; ribosomes

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have identified several essential, conserved proteins that are present in 66S pre-ribosomes and necessary for ribosome assembly. In order to identify neighbors of these proteins in assembling ribosomes or to explore for moonlighting partners, we have begun screening the P. James library for partners of each protein. We would also like to collaborate with the YRC to screen your ordered two-hybrid library, using each of these proteins as bait.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。我们已经确定了几个基本的，保守的蛋白质，存在于66 S前核糖体和核糖体组装所必需的。为了确定这些蛋白质在组装核糖体中的邻居或探索兼职伙伴，我们已经开始筛选P. James文库中的每种蛋白质的伙伴。我们也希望与YRC合作，使用这些蛋白质中的每一种作为诱饵，筛选您订购的双杂交文库。