TWO-HYBRID IDENTIFICATION OF PROTEINS THAT RECOGNIZE THE UBIQUITIN LIKE MODIFIE

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。在酵母细胞中，丝状生长和其他几个过程需要一个研究很少的泛素样系统。特别是，在丝状生长过程中，细胞伸长需要泛素样修饰物Urm1和E1酶Uba4。缺乏这两种蛋白的突变体也对某些有机氧化剂和雷帕霉素敏感。推测的E2和E3酶的“熊果化”途径尚未确定。同样，识别已被糖化的目标的细胞蛋白质也没有被识别出来。我们提出了一个使用Urm1-Gal4BD结构的双杂交筛选来识别这些蛋白质。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A little studied ubiquitin-like system is required for filamentous growth and several other processes in yeast cells. In particular, the ubiquitin-like modifier Urm1 and the E1 enzyme Uba4 are required for cell elongation during filamentous growth. Mutants lacking either of these proteins are also sensitive to some organic oxidizing agents and to rapamycin. Putative E2 and E3 enzymes for the "urmylation" pathway have not been identified. Likewise, cellular proteins that recognize a target that has been urmylated have not been identified. We propose a two-hybrid screen using a Urm1-Gal4 BD construct to identify such proteins.