TWO-HYBRID IDENTIFICATION OF PROTEINS THAT RECOGNIZE THE UBIQUITIN LIKE MODIFIE

识别泛素样修饰蛋白的两种杂交鉴定

• 批准号: 7420734
• 负责人: GEORGE F. SPRAGUE
• 金额: $ 0.5万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-20 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7420734
• 关键词: enzyme mechanism; enzymes; mutant; oxidizing agents; proteins; ubiquitin; yeasts

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A little studied ubiquitin-like system is required for filamentous growth and several other processes in yeast cells. In particular, the ubiquitin-like modifier Urm1 and the E1 enzyme Uba4 are required for cell elongation during filamentous growth. Mutants lacking either of these proteins are also sensitive to some organic oxidizing agents and to rapamycin. Putative E2 and E3 enzymes for the "urmylation" pathway have not been identified. Likewise, cellular proteins that recognize a target that has been urmylated have not been identified. We propose a two-hybrid screen using a Urm1-Gal4 BD construct to identify such proteins.

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。酵母细胞的丝状生长和其他几个过程需要一个研究很少的泛素样系统。特别是，泛素样修饰物Urm1和E1酶Uba4是丝状生长过程中细胞伸长所必需的。缺乏这两种蛋白质中的任何一种的突变体也对某些有机氧化剂和雷帕霉素敏感。目前尚未确定“未甲基化”途径的E2和E3酶。同样地，细胞蛋白识别的目标，已被未mylated尚未确定。我们提出使用Urm1-Gal4 BD结构的双杂交筛选来鉴定这些蛋白质。