DIFFERENTIATION OF ALPHA- VS BETA- ASPARTIC ACID USING ETD

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。我们最近成功地证明了ECD可以很容易地区分多肽和蛋白质中的α-和β-天冬氨酸残基，这提出了一个明显的问题，即同样的方法是否可以用于电子转移解离(ETD)。但是，资源中不存在ETD指令插入。因此，在ASMS 2005年之后，在普渡与Scott McLuckey教授的团队开始了一项合作。特定的标记多肽的样本被送往普渡大学，并返回光谱，这表明这项技术确实适用于ETD。手稿目前正在撰写中。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our recent success in showing that ECD can easily differentiate alpha- and beta- aspartic acid residues in peptides and proteins raised the obvious question as to whether or not the same methods could be used with Electron Transfer Dissociation (ETD). However, ETD instrumentation does not exist at the resource. Thus, following ASMS 2005, a collaboration was started with Prof. Scott McLuckey's group in Purdue. Samples of specific, labeled peptides were sent to Purdue, and spectra were returned which shows that this technique does indeed work with ETD as well. The manuscript is currently being written.