DIFFERENTIATION OF ALPHA- VS BETA- ASPARTIC ACID USING ETD

使用 ETD 区分 α- 天冬氨酸和 β- 天冬氨酸

• 批准号: 7369303
• 负责人: PETER B. O'CONNOR
• 金额: $ 0.03万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7369303
• 关键词: DIFFERENTIATION; ALPHA; VS; BETA; ASPARTIC

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our recent success in showing that ECD can easily differentiate alpha- and beta- aspartic acid residues in peptides and proteins raised the obvious question as to whether or not the same methods could be used with Electron Transfer Dissociation (ETD). However, ETD instrumentation does not exist at the resource. Thus, following ASMS 2005, a collaboration was started with Prof. Scott McLuckey's group in Purdue. Samples of specific, labeled peptides were sent to Purdue, and spectra were returned which shows that this technique does indeed work with ETD as well. The manuscript is currently being written.

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。我们最近成功地表明ECD可以很容易地区分肽和蛋白质中的α -和β -天冬氨酸残基，这提出了一个明显的问题，即电子转移解离（ETD）是否可以使用相同的方法。然而，资源中不存在ETD工具。因此，在asm 2005之后，与普渡大学Scott McLuckey教授的小组开始了合作。特定的、标记的肽的样品被送到普渡大学，并返回光谱，这表明这种技术确实也适用于ETD。手稿目前正在撰写中。