TOTAL OXIDATIVE POST TRANSLATIONAL MODIFICATION MAP OF H-RAS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。该项目旨在利用我们最近开发的ESI-QQQ-FTMS的独特功能来充分表征氧化应激条件下发生的H-RAS的翻译后修饰。该项目将使用一系列技术，包括自上而下和自下而上的蛋白质组学方法、选择性积累、Q2-CAD和ECD。所涉及的样品是商业购买的H-RAS，既有天然状态的，也有用过氧亚硝酸根进行氧化修饰的。这将首先在体外完成，但目标是一旦体外修饰表征后，将其扩展到体内模型。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project aims to use the unique capabilities of our recently developed ESI-qQq-FTMS to fully characterize the post-translational modifications of H-RAS that occur during oxidative stress conditions. The project will use a combination of techniques including top-down and bottom-up proteomics methods, selective accumulation, Q2-CAD, and ECD. The samples involved are commercially purchased H-RAS, both native state and oxidatively modified with peroxynitrite. This will be done in-vitro initially, but the goal is to extend this to in-vivo models once the in-vitro modifications are characterized.