SELECTIVE IN VIVO VISUALIZATION OF COLLAGEN & ELASTIN BY MPM

胶原蛋白的选择性体内可视化

• 批准号: 7365599
• 负责人: Bruce J. Tromberg
• 金额: $ 0.39万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7365599
• 关键词: SELECTIVE; VIVO; VISUALIZATION; COLLAGEN; ELASTIN

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The mechanical properties of a blood vessel are important determinants of the pressure-flow relationship, the speed of pulse waves in the vessels, the stress distribution in the vessel wall, and the phenomena of mass transport through the arterial wall. The mechanical properties of blood vessels are derived from collagen and elastin fibers, smooth muscle cells, and ground substances. In the present proposal, we will determine the micro-structural basis for the mechanical properties of coronary blood vessel. We will combine tri-axial mechanical testing (inflation, stretch and torsion) of the coronary arteries with the simultaneous determination of collagen and elastin structure. We will use two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) microscopy as a high-resolution biological imaging technique to selectively monitor the structural changes of collagen and elastin in response to different physical loading conditions. Determination of the origin of signal from collagen and elastin by using spectral measurements allows the isolation of these arterial tissue components by means of spectral filtering, and generates high-contrast images of the vessels. This research will lay a micro-structural foundation for the observed mechanical properties of blood vessels which may serve as a tool for diagnosis in pathology.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。血管的机械性质是压力-流量关系、血管中脉搏波的速度、血管壁中的应力分布以及通过动脉壁的质量输送现象的重要决定因素。血管的机械性质来自胶原蛋白和弹性蛋白纤维、平滑肌细胞和基质。在本建议中，我们将确定冠状血管的力学性能的微观结构基础。我们将结合联合收割机三轴力学测试（膨胀，拉伸和扭转）的冠状动脉与胶原蛋白和弹性蛋白结构的同时测定。我们将使用双光子激发荧光（TPEF）和二次谐波发生（SHG）显微镜作为一种高分辨率的生物成像技术，选择性地监测胶原蛋白和弹性蛋白的结构变化，以响应不同的物理负载条件。通过使用光谱测量来确定来自胶原蛋白和弹性蛋白的信号的来源允许通过光谱滤波来分离这些动脉组织成分，并且生成血管的高对比度图像。本研究将为观察血管的力学性质奠定微观结构基础，从而为病理诊断提供工具。