Imaging Core

项目摘要/摘要 成像核心的广泛目标是使用尖端光学成像技术非侵入性地 想象一下皮肤生物学和疾病背后的宏观、微观和分子过程。这个 核心建立在贝克曼激光研究所和医疗诊所(BLI)和 荧光动力学实验室(LFD)，加州大学欧文分校的长期中心，开创了 生物和医学光学和光子学技术的发展和应用。BLI和LFD将 创建新的皮肤成像套件，其中包含P30皮肤的特定、最先进的活体光学成像平台 生物研究人员，包括：多光子显微镜、荧光寿命成像显微镜、 薄膜；相干拉曼散射；空间频域成像；激光散斑成像， LSI；和光学相干层析成像(OCT)；以及计算方法和模型 了解光在皮肤中的传播(即皮肤光学)。这些技术被用来可视化荧光 分子探针和报告器以及在细胞、ECM 和血管系统。通过采用这些多模式、多尺度技术，成像核心将促进 这些研究可以带来强大的新皮肤生物学见解，并可以移植到人类身上。这是 通过将成像数据集与基因组学和系统生物学核心相结合来开发 然后可以在生物实验中进行测试的模型，包括在动物身上。除了提供切割 用于研究的边缘技术，成像核心的一个关键组成部分将是提供培训资源， 推动和支持跨学科活动的传播和合作。我们的目标是将 UCI和皮肤研究部门的技术开发人员、建模专家、生物学家和临床医生 建立团队，共享知识并应用新工具来解决长期存在的问题 有问题。这将通过创建实践培训、授课培训和 为皮肤生物学研究人员提供知识、资源和支持的传播活动 需要将最先进的光学成像技术整合到皮肤生物学研究中。最后，我们的技术将 继续优化，从社区反馈，以更好地了解皮肤生物学和 病理生理学，最终目标是开发新的方法来改善患者的预后 患有皮肤病的。

PROJECT SUMMARY/ABSTRACT The broad objective of the Imaging Core is to use cutting edge optical imaging technologies to non-invasively visualize the macroscopic, microscopic, and molecular processes that underlie skin biology and disease. The Core builds on technologies and expertise in the Beckman Laser Institute and Medical Clinic (BLI) and the Laboratory for Fluorescence Dynamics (LFD), longstanding UC Irvine centers that have pioneered the development and application of optics and photonics technologies for biology and medicine. BLI and LFD will create a new skin imaging suite housing specific, state-of-the-art, in vivo optical imaging platforms to P30 skin biology investigators, including: Multiphoton Microscopy, MPM; Fluorescence Lifetime Imaging Microscopy, FLIM; Coherent Raman Scattering, CRS; Spatial Frequency Domain Imaging, SFDI; Laser Speckle Imaging, LSI; and Optical Coherence Tomography (OCT); as well as computational methods and models for understanding light propagation in skin (i.e. skin optics). These technologies are used to visualize fluorescent molecular probes and reporters as well as provide label-free contrast from intrinsic tissue signals in cells, ECM, and vasculature. By employing these multi-modal, multi-scale technologies, the imaging core will facilitate studies that can lead to powerful new skin biology insights that can be translated to humans. This is accomplished by integrating imaging datasets with the Genomics and Systems biology cores to develop models that can then be tested in biological experiments, including in animals. In addition to providing cutting edge technologies for research, a key component of the Imaging Core will be to provide resources for training, dissemination, and collaboration that drive and support interdisciplinary activities. Our goal is to bring together technology developers, modeling experts, biologists, and clinicians both within UCI and the skin research community at large to build teams that can share knowledge and apply new tools to solve longstanding problems. This will be accomplished by creating a combination of hands-on training, didactic training, and dissemination activities that provide skin biology researchers with the knowledge, resources, and support needed to integrate state-of the-art optical imaging into skin biology research. Finally, our technologies will continue to be optimized, with feedback from the community, to better understand skin biology and pathophysiology, with the ultimate goal of developing new approaches that improve outcomes for patients suffering from cutaneous disease.