STRUCTURAL STUDIES ON MAMMALIAN AND BACTERIAL BINDING PROTEINS AND ENZYMES

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。我们实验室的一般研究领域包括确定具有生物医学和工业重要性的蛋白质和酶的三维结构。在SSRL上更新BL7-1和BL9-2的光束时间主要集中在收集哺乳动物和细菌结合和运输蛋白的高分辨率衍射数据，以及涉及叶酸代谢，细菌细胞壁生物合成，抗生素耐药性和二硫键异构化的一些酶。在SSRL收集数据的愿望是基于我们对来自许多不同晶体的高质量，高分辨率衍射数据的需求，其中一些晶体对于传统的x射线源来说太小或太敏感，或者有很长的细胞边缘。此外，我们现在生产的大部分蛋白质都是硒-蛋氨酸衍生物，我们预计对收集准确的MAD数据的需求会增加。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The general area of research in our laboratory involves the determination of the 3-D structures of proteins and enzymes of biomedical and industrial importance. This renewal for beam time on BL7-1 and BL9-2 at SSRL focuses on the collection of high resolution diffraction data from mammalian and bacterial binding and transport proteins, along with a number of enzymes involved in folate metabolism, bacterial cell wall biosynthesis, antibiotic resistance and disulfide bond isomerization. The desire to collect data at SSRL is based upon our need for high quality, high resolution diffraction data from a number of different crystals, some of which are either too small or sensitive for conventional x-ray sources, or have long cell edges. In addition, we are now producing the majority of our proteins as seleno-methionine derivatives, and we envisage an increased requirement for the collection of accurate MAD data.