STRUCTURAL STUDIES ON MAMMALIAN AND BACTERIAL BINDING PROTEINS AND ENZYMES

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在我们的实验室研究的一般领域涉及的生物医学和工业重要性的蛋白质和酶的3-D结构的测定。 SSRL对BL 7 -1和BL 9 -2的光束时间更新的重点是从哺乳动物和细菌结合和转运蛋白收集高分辨率衍射数据，沿着许多参与叶酸代谢、细菌细胞壁生物合成、抗生素抗性和二硫键异构化的酶。 在SSRL收集数据的愿望是基于我们对来自许多不同晶体的高质量、高分辨率衍射数据的需求，其中一些晶体对于传统的X射线源来说太小或太敏感，或者具有长的单元边缘。 此外，我们现在生产的大多数蛋白质作为硒蛋氨酸衍生物，我们设想一个准确的MAD数据收集的需求增加。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The general area of research in our laboratory involves the determination of the 3-D structures of proteins and enzymes of biomedical and industrial importance. This renewal for beam time on BL7-1 and BL9-2 at SSRL focuses on the collection of high resolution diffraction data from mammalian and bacterial binding and transport proteins, along with a number of enzymes involved in folate metabolism, bacterial cell wall biosynthesis, antibiotic resistance and disulfide bond isomerization. The desire to collect data at SSRL is based upon our need for high quality, high resolution diffraction data from a number of different crystals, some of which are either too small or sensitive for conventional x-ray sources, or have long cell edges. In addition, we are now producing the majority of our proteins as seleno-methionine derivatives, and we envisage an increased requirement for the collection of accurate MAD data.