MODE OF ACTION OF GROWTH ENHANCES AND CHELATING COMPOUNDS ON THE UPTAKE OF CD AN

生长促进剂和螯合化合物对 CD AN 摄取的作用方式

• 批准号: 7370541
• 负责人: JORGE GARDEA-TORRESDEY
• 金额: $ 0.6万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7370541
• 关键词: MODE; ACTION; GROWTH; ENHANCES; CHELATING

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The remediation of heavy metals from the environment using plants is a novel technology commonly termed phytoremediation. Many plants have shown the ability of uptake heavy metals, including lead and cadmium, from contaminated growth media. However, the addition of chelating agents such as EDTA has been shown to increase the uptake and translocation of both cadmium and lead in many different plant species. We have recently been investigating the use of growth promoters such as gibberlic acid, indolic-3-acetic acid, kinetine, 2,4-dichlorophenoxy acid, and glutathione, with mixtures of EDTA. We have observed that specific molar combinations of EDTA and IAA increase the uptake of lead in alfalfa plants by approximately 2800% over treatments with lead alone in the leaves of the plants. In addition, the use of equal molar concentrations of indole-3-acetic acid and EDTA increased the uptake of lead by 600% over the use of EDTA alone in the leaves of alfalfa plants. However, in the roots and stems of the alfalfa plants the uptake of lead was reduced when combinations of indole-3-acetic acid-EDTA were used. These results have much promise in producing a viable phytoremediation system. We plan to use XAS to understand the chemistry and subsequently, the mode of action that growth promoters/ enhancers have on the uptake and coordination of both lead and cadmium in live plants. XAS studies will provide us with a chemical understanding of this complex biochemical process of reducing the toxicity of the metals and at the same time increasing the translocation of metals through the plant.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。使用植物从环境中对重金属进行修复是一种通常称为植物修复的新技术。许多植物已经显示出污染的生长培养基中摄取重金属（包括铅和镉）的能力。然而，已经显示出螯合剂（例如EDTA）的添加可增加许多不同植物物种中镉和铅的摄取和易位。最近，我们一直在研究使用EDTA的混合物，研究了生长启动子，例如甘贝尔酸，吲哚-3-乙酸，动力基，2,4-二氯苯氧基酸和谷胱甘肽。我们已经观察到，EDTA和IAA的特定摩尔组合增加了苜蓿植物中铅的摄取，而仅在植物叶片中单独使用铅的处理方法约为2800％。另外，在苜蓿植物的叶片中，仅使用相等摩尔浓度的吲哚-3-乙酸和EDTA增加了铅的吸收600％。然而，在使用吲哚-3-乙酸 - EDTA的组合时，在苜蓿植物的根和茎中减少了铅的吸收。这些结果在产生可行的植物修复系统方面具有很大的希望。我们计划使用XAS了解化学，随后，生长启动子/增强剂对活植物中铅和镉的吸收和协调的作用方式。 XAS研究将为我们提供对这种复杂的生化过程的化学理解，以降低金属的毒性，同时增加金属通过植物的易位。