MODE OF ACTION OF GROWTH ENHANCES AND CHELATING COMPOUNDS ON THE UPTAKE OF CD AN

生长促进剂和螯合化合物对 CD AN 摄取的作用方式

• 批准号: 7370541
• 负责人: JORGE GARDEA-TORRESDEY
• 金额: $ 0.6万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7370541
• 关键词: MODE; ACTION; GROWTH; ENHANCES; CHELATING

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The remediation of heavy metals from the environment using plants is a novel technology commonly termed phytoremediation. Many plants have shown the ability of uptake heavy metals, including lead and cadmium, from contaminated growth media. However, the addition of chelating agents such as EDTA has been shown to increase the uptake and translocation of both cadmium and lead in many different plant species. We have recently been investigating the use of growth promoters such as gibberlic acid, indolic-3-acetic acid, kinetine, 2,4-dichlorophenoxy acid, and glutathione, with mixtures of EDTA. We have observed that specific molar combinations of EDTA and IAA increase the uptake of lead in alfalfa plants by approximately 2800% over treatments with lead alone in the leaves of the plants. In addition, the use of equal molar concentrations of indole-3-acetic acid and EDTA increased the uptake of lead by 600% over the use of EDTA alone in the leaves of alfalfa plants. However, in the roots and stems of the alfalfa plants the uptake of lead was reduced when combinations of indole-3-acetic acid-EDTA were used. These results have much promise in producing a viable phytoremediation system. We plan to use XAS to understand the chemistry and subsequently, the mode of action that growth promoters/ enhancers have on the uptake and coordination of both lead and cadmium in live plants. XAS studies will provide us with a chemical understanding of this complex biochemical process of reducing the toxicity of the metals and at the same time increasing the translocation of metals through the plant.

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。利用植物修复环境中的重金属是一种新兴的技术，通常被称为植物修复。许多植物已经显示出从受污染的生长介质中吸收重金属的能力，包括铅和镉。然而，在许多不同的植物物种中，添加螯合剂（如EDTA）已被证明可以增加镉和铅的吸收和转运。我们最近一直在研究生长促进剂如赤霉素、吲哚-3-乙酸、酪氨酸、2,4-二氯苯氧酸和谷胱甘肽与EDTA混合物的使用情况。我们观察到EDTA和IAA的特定摩尔组合增加了苜蓿植物对铅的吸收，比单独使用铅的处理增加了约2800%。此外，使用等摩尔浓度的吲哚-3-乙酸和EDTA比单独使用EDTA在苜蓿叶片中增加了600%的铅吸收率。然而，在紫花苜蓿植物的根和茎中，当使用吲哚-3-乙酸- edta组合时，铅的吸收减少。这些结果为建立可行的植物修复系统提供了很大的希望。我们计划使用XAS来了解生长促进剂/增强剂对活植物中铅和镉的吸收和协调的化学作用，以及随后的作用模式。XAS研究将为我们提供对这一复杂生化过程的化学理解，该过程降低了金属的毒性，同时增加了金属在植物中的转运。