MODE OF ACTION OF GROWTH ENHANCES AND CHELATING COMPOUNDS ON THE UPTAKE OF CD AN

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 利用植物修复环境中的重金属污染是一项新兴的技术，通常被称为植物修复。 许多植物已经显示出从污染的生长介质中吸收重金属的能力，包括铅和镉。 然而，添加螯合剂如EDTA已被证明可以增加许多不同植物物种对镉和铅的吸收和转运。 我们最近一直在研究使用生长促进剂，如赤霉酸，吲哚-3-乙酸，激酶，2，4-二氯苯氧基酸，谷胱甘肽，与EDTA的混合物。 我们已经观察到，特定的EDTA和IAA的摩尔组合增加了约2800%的铅在苜蓿植物中的植物叶片中的铅单独处理的吸收。 此外，使用等摩尔浓度的吲哚-3-乙酸和EDTA增加了600%以上的单独使用EDTA在苜蓿植物的叶子中的铅的吸收。 然而，在根和茎的苜蓿植物的铅的吸收减少时，吲哚-3-乙酸-EDTA的组合使用。 这些结果有很大的希望，在生产一个可行的植物修复系统。 我们计划使用XAS来了解化学，随后，生长促进剂/增强剂对活植物中铅和镉的吸收和协调的作用模式。 XAS的研究将为我们提供一个化学的理解，这个复杂的生物化学过程，减少金属的毒性，同时增加金属通过植物的转运。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The remediation of heavy metals from the environment using plants is a novel technology commonly termed phytoremediation. Many plants have shown the ability of uptake heavy metals, including lead and cadmium, from contaminated growth media. However, the addition of chelating agents such as EDTA has been shown to increase the uptake and translocation of both cadmium and lead in many different plant species. We have recently been investigating the use of growth promoters such as gibberlic acid, indolic-3-acetic acid, kinetine, 2,4-dichlorophenoxy acid, and glutathione, with mixtures of EDTA. We have observed that specific molar combinations of EDTA and IAA increase the uptake of lead in alfalfa plants by approximately 2800% over treatments with lead alone in the leaves of the plants. In addition, the use of equal molar concentrations of indole-3-acetic acid and EDTA increased the uptake of lead by 600% over the use of EDTA alone in the leaves of alfalfa plants. However, in the roots and stems of the alfalfa plants the uptake of lead was reduced when combinations of indole-3-acetic acid-EDTA were used. These results have much promise in producing a viable phytoremediation system. We plan to use XAS to understand the chemistry and subsequently, the mode of action that growth promoters/ enhancers have on the uptake and coordination of both lead and cadmium in live plants. XAS studies will provide us with a chemical understanding of this complex biochemical process of reducing the toxicity of the metals and at the same time increasing the translocation of metals through the plant.