MODE OF ACTION OF GROWTH ENHANCES AND CHELATING COMPOUNDS ON THE UPTAKE OF CD AN

生长促进剂和螯合化合物对 CD AN 摄取的作用方式

• 批准号: 7954217
• 负责人: JORGE GARDEA-TORRESDEY
• 金额: $ 0.27万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954217
• 关键词: Acetic Acids; Acids; Alfalfa; Biochemical Process; Cadmium; Chelating Agents; Chemicals; Chemistry; Complex; Computer Retrieval of Information on Scientific Projects Database; Culture Media; Edetic Acid; Enhancers; Environment; Funding; Glutathione; Grant; Growth; Heavy Metals; Institution; Lead; Left; Life; Metals; Plant Leaves; Plant Roots; Plants; Research; Research Personnel; Resources; Source; System; Time; United States National Institutes of Health; indoleacetic acid; metal poisoning; new technology; promoter; remediation; stem; structural biology; synchrotron radiation; uptake

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The remediation of heavy metals from the environment using plants is a novel technology commonly termed phytoremediation. Many plants have shown the ability of uptake heavy metals, including lead and cadmium, from contaminated growth media. However, the addition of chelating agents such as EDTA has been shown to increase the uptake and translocation of both cadmium and lead in many different plant species. We have recently been investigating the use of growth promoters such as gibberlic acid, indolic-3-acetic acid, kinetine, 2,4-dichlorophenoxy acid, and glutathione, with mixtures of EDTA. We have observed that specific molar combinations of EDTA and IAA increase the uptake of lead in alfalfa plants by approximately 2800% over treatments with lead alone in the leaves of the plants. In addition, the use of equal molar concentrations of indole-3-acetic acid and EDTA increased the uptake of lead by 600% over the use of EDTA alone in the leaves of alfalfa plants. However, in the roots and stems of the alfalfa plants the uptake of lead was reduced when combinations of indole-3-acetic acid-EDTA were used. These results have much promise in producing a viable phytoremediation system. We plan to use XAS to understand the chemistry and subsequently, the mode of action that growth promoters/ enhancers have on the uptake and coordination of both lead and cadmium in live plants. XAS studies will provide us with a chemical understanding of this complex biochemical process of reducing the toxicity of the metals and at the same time increasing the translocation of metals through the plant.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 利用植物修复环境中的重金属是一种通常称为植物修复的新技术。 许多植物表现出从受污染的生长介质中吸收重金属的能力，包括铅和镉。 然而，添加 EDTA 等螯合剂已被证明可以增加许多不同植物物种对镉和铅的吸收和转运。 我们最近一直在研究生长促进剂的使用，例如赤霉酸、吲哚-3-乙酸、激动剂、2,4-二氯苯氧基酸和谷胱甘肽以及 EDTA 的混合物。 我们观察到，与单独使用铅处理相比，EDTA 和 IAA 的特定摩尔组合可使苜蓿植物对铅的吸收增加约 2800%。 此外，与单独使用 EDTA 相比，使用等摩尔浓度的 3-吲哚-3-乙酸和 EDTA 使苜蓿植物的叶子对铅的吸收增加了 600%。 然而，当使用吲哚-3-乙酸-EDTA 组合时，苜蓿植物的根和茎中铅的吸收量会减少。 这些结果对于产生可行的植物修复系统很有希望。 我们计划使用 XAS 来了解化学性质，以及生长促进剂/增强剂对活体植物中铅和镉的吸收和协调的作用模式。 XAS 研究将为我们提供对这种降低金属毒性并同时增加金属在植物中的易位的复杂生化过程的化学理解。