EFFECTS OF CIGARETTE SMOKE ON CYCLOXYGENASE-2 IN ORAL MUCOSA

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。主要假设：本研究的主要假设是健康的重度吸烟者在口腔黏膜中表达较高水平的环氧化酶-2 （COX-2）及相关分子。具体目标：1；评估30名重度吸烟者和30名从不吸烟者正常颊（颊）粘膜（颊表面）中COX-2水平的差异。2. 评价30例从不吸烟者的正常口腔黏膜中COX-2水平与卷烟包装年限的关系。3. 评估40例重度吸烟者和40例从不吸烟者正常颊（颊）粘膜（颊表面）表皮生长因子受体（EGFR）酪氨酸激酶及其他相关分子激活水平的差异。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Primary Hypothesis: The primary hypothesis of this study is that healthy heavy smokers will express higher levels of cyclooxygenase-2 (COX-2) and related molecules in their oral mucosa. Specific Aims: 1. To evaluate the difference in levels of COX-2 in normal appearing buccal (cheek) mucosa (surface lining of the cheek) between 30 heavy smokers and 30 never smokers. 2. To evaluate the association of pack years of cigarette exposure with levels of COX-2 in normal appearing buccal mucosa of 30 never smokers. 3. To evaluate the difference in levels of activation of epidermal growth factor receptor (EGFR) tyrosine kinase and other related molecules in normal appearing buccal (cheek) mucosa (surface lining of the cheek) between 40 heavy smokers and 40 never smokers.