UNDERSTANDING THE PATHOGENESIS OF CAMPYLOBACTER JEJUNI

了解空肠弯曲菌的发病机制

• 批准号: 7359131
• 负责人: Victor J. DiRita
• 金额: $ 8.13万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7359131
• 关键词: UNDERSTANDING; PATHOGENESIS; CAMPYLOBACTER; JEJUNI

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our long objectives are to understand the unique biology and pathogenicity of Campylobacter jejuni, an important human pathogen and commensal of food species including the chicken, from which the majority of human cases originate (Centers for Disease Control). We developed a number of essential genetic tools previously unavailable for the study of this microbe, including random and site specific mutagenesis mutagenesis systems, reporter plasmids for studying transcription regulation and shuttle/expression vectors (Hendrixson, et al., 2001; Hendrixson, O¿Rourke and DiRita, unpublished). We have used these to identify and characterize genes required for colonization of chickens, flagellar motility, cell invasion, and competence for DNA transformation (Hendrixson, et al., 2001; Hendrixson and DiRita, unpublished, Fogg and DiRita, unpublished, Wiesner, Hendrixson and DiRita, in preparation). Interaction data and protein expression profiling data will both be very helpful to our studies on C. jejuni. Different ongoing projects relate to identifying and characterizing protein complexes or assessing the protein profiles of key mutants. High-throughput cloning technology as proposed, leaving the individual clones tagged for protein purification, will be of enormous value to my group as it would obviate the need for us to produce tagged proteins for purification on an individual basis.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。我们的长期目标是了解空肠弯曲菌的独特生物学和致病性，空肠弯曲菌是一种重要的人类病原体，也是包括鸡在内的食物物种的主要来源，大多数人类病例都来自鸡（疾病控制中心）。我们开发了许多以前无法用于研究这种微生物的基本遗传工具，包括随机和位点特异性诱变诱变系统、用于研究转录调节的报告质粒和穿梭/表达载体（Hendrixson等人，2001年; Hendrixson、O â Rourke和DiRita，未发表）。我们已经使用这些来鉴定和表征鸡定殖、鞭毛运动、细胞侵入和DNA转化能力所需的基因（Hendrixson，et al.，2001; Hendrixson和DiRita，未出版，Fogg和DiRita，未出版，Wiesner，Hendrixson和DiRita，正在编写）。 相互作用数据和蛋白质表达谱数据将对我们的研究有很大的帮助。空肠。不同的正在进行的项目涉及识别和表征蛋白质复合物或评估关键突变体的蛋白质谱。所提出的高通量克隆技术，将单个克隆标记用于蛋白质纯化，对我的小组将具有巨大的价值，因为它将使我们不需要在单个基础上生产用于纯化的标记蛋白质。