Structure of ToxR/S and TcpP/H: Virulence Regulators

ToxR/S 和 TcpP/H 的结构：毒力调节剂

• 批准号: 6601794
• 负责人: Victor J. DiRita
• 金额: $ 7.63万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6601794
• 关键词: DNA binding protein; Vibrio cholerae; bacterial proteins; binding sites; cholera toxin; crystallization; gene expression; genetic transcription; membrane proteins; method development; pilus; protein binding; protein protein interaction; protein purification; protein structure function; virulence

DESCRIPTION (provided by applicant): This proposal seeks to develop methods for overexpressing, purifying and crystallizing membrane-localized transcription control proteins from Vibrio cholerae, the agent of human cholera disease. ToxR and TcpP are bi-topic membrane proteins with cytoplasmic DNA binding/activation domains. They control expression of important virulence factors by virtue of their ability to activate expression of toxT, the activator of genes encoding cholera toxin and toxin-coregulated pilus. The DNA binding/activation domains of ToxR and TcpP are homologous to the winged Helix-Turn-Helix (w-HTH) family of proteins. The structures of other w-HTH domains has been solved, but the unusual membrane topology of ToxR and TcpP, and significant preliminary data aimed at determining how they recognize DNA and activate toxT transcription, compel an interest in solving their crystal structures when bound to operator DNA. Upon completion, this study will enable us to discriminate between distinct hypotheses for how ToxR and TcpP function. Along with determining the structure of ToxR and TcpP, of interest also is overexpression, purification and crystallization of the structures of membrane effector proteins required for the activity of each: ToxS in the case of ToxR, and TcpH in the case of TcpP. The specific aims of the proposal are as follows: (i.) To purify a 6-His tagged version of full length ToxR for crystallization and structural determination with and without its toxT promoter binding site or its binding site in the ompU promoter (a promoter activated by ToxR independently of TcpP) (ii.) To purify a 6-His tagged version of full length TcpP for crystallization and structural determination with and without its toxT promoter-binding site (iii.) To obtain structural data on co-crystals of ToxR and TcpP on the toxT promoter (to determine whether protein-protein interactions affect DNA binding) (iv.) To purify FLAG epitope- tagged versions of ToxS and TcpH for crystallization and structural determination alone or in a co-crystal with ToxR or TcpP

描述（由申请人提供）：本提案寻求开发用于过表达、纯化和结晶来自霍乱弧菌（人类霍乱病的病原体）的膜定位转录控制蛋白的方法。ToxR和TcpP是具有细胞质DNA结合/活化结构域的双位膜蛋白。它们通过激活toxT表达的能力控制重要毒力因子的表达，toxT是编码霍乱毒素和毒素共调节菌毛的基因的激活剂。ToxR和TcpP的DNA结合/活化结构域与有翼螺旋-转向-受阻（w-HTH）蛋白家族同源。其他w-HTH结构域的结构已经得到解决，但ToxR和TcpP的不寻常的膜拓扑结构，以及旨在确定它们如何识别DNA并激活toxT转录的重要初步数据，迫使人们有兴趣解决它们与操纵基因DNA结合时的晶体结构。完成后，这项研究将使我们能够区分ToxR和TcpP功能的不同假设。沿着确定ToxR和TcpP的结构，还感兴趣的是每种活性所需的膜效应蛋白的结构的过表达、纯化和结晶：ToxR的情况下是ToxS，TcpP的情况下是TcpH。 建议的具体目标如下：（i）纯化全长ToxR的6-His标记形式用于结晶和结构测定，其具有和不具有其toxT启动子结合位点或其在ompU启动子（由ToxR独立于TcpP激活的启动子）中的结合位点（ii.）为了纯化全长TcpP的6-His标记形式用于结晶和结构测定，具有和不具有其toxT启动子结合位点（iii.）为了获得toxT启动子上ToxR和TcpP的共晶体的结构数据（以确定蛋白质-蛋白质相互作用是否影响DNA结合）（iv.）纯化FLAG表位标记形式的ToxS和TcpH，用于单独或与ToxR或TcpP共晶体中的结晶和结构测定