CRYSTAL STRUCTURE OF MEND

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。(1R 6 R）-2-琥珀酰基-6-羟基-2，4-环己二烯-1-羧酸（SHCHC）合酶，也称为MenD，参与甲萘醌（维生素K2）生物合成途径。该酶是ThDP依赖性酶超家族的一部分，然而它是已知催化ThDP加合物与不饱和羧酸酯的α-碳的Stetter样1，4-加成的唯一酶。这种酶的晶体结构对于阐明这种重要酶的作用机制具有重要意义。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. (1R,6R)-2-Succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC) synthase, also called MenD, participates in the menaquinone (Vitamin K2) biosynthetic pathway. The enzyme is a part of the superfamily of ThDP-dependent enzymes, however it is the only enzyme known to catalyze a Stetter-like 1,4-addition of a ThDP adduct to the alpha-carbon of an unsaturated carboxylate. The crystal structure of this enzyme will be important for clarifying the mechanism of this important enzyme.