STRUCTURAL STUDIES OF NOVEL CARBOHYDRATE MODIFYING ENZYMES AND PROTEIN COMPLEXES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我实验室的工作重点是新型酶和蛋白质复合体的结构表征。我们目前正在为这些研究寻找几种不同的酶/系统。 我们在新型酶方面的工作是与大卫·帕尔默博士(UofS)合作进行的。我们与他的团队正在进行两个项目，以确定维生素K生物合成途径中的重要酶*2-Succinyl-6-hydroxy-2，4-cyclohexadiene-1-carboxylate合成酶(MEND)*的结构，以及肌醇脱氢酶(IDH)*，一种具有不同底物识别能力的蛋白质。 蛋白质之间的相互作用对于所有细胞系统的正常运作是必不可少的。一般来说，有两种类型的蛋白质-蛋白质相互作用，紧密结合系统，如抗体所见：抗原相互作用和蛋白质复合体的形成，以及瞬时或弱结合作用，如在酶过程中相互作用的两个蛋白质之间。我们目前正在研究的蛋白质-蛋白质相互作用体系是硫氧还蛋白体系。我们目前的研究目标是探索不同物种的硫氧还蛋白(Trx)和硫氧还蛋白还原酶(TrxR)之间的相互作用，表征对结合至关重要的相互作用，并研究这些酶补偿温差和其他进化压力的方式，以保持它们相互作用的能力。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The work in my laboratory is focused on the structural characterization of novel enzymes and protein complexes. We are currently looking at several different enzymes/systems for these studies. Our work on novel enzymes is being conducted in collaboration with Dr. David Palmer (UofS). We have two projects underway with his group, to determine the structures of *2-Succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC) synthase (MenD)*, and important enzyme in the vitamin K biosynthetic pathway, and *inositol dehydrogenase (IDH)*, a protein that has diverse substrate recognition. Protein-protein interactions are essential for the proper functioning of all cellular systems. In general, there are two types of protein-protein interactions, tight-binding systems such as those seen with antibody:antigen interactions and the formation of protein complexes, and transient or weak binding interactions such as are seen between two proteins interacting in an enzymatic process. The protein-protein interaction system that we are currently studying is the thioredoxin system. Our current research objectives are to probe the interactions between thioredoxin (Trx) and thioredoxin reductase (TrxR) from different species, to characterize the interactions that are important for binding and to examine the way these enzymes are compensating for temperature differences and other evolutionary pressures to maintain their ability to interact with each other.