STRUCTURAL STUDIES OF NOVEL CARBOHYDRATE MODIFYING ENZYMES AND PROTEIN COMPLEXES

新型碳水化合物修饰酶和蛋白质复合物的结构研究

• 批准号: 7955113
• 负责人: DAVID A SANDERS
• 金额: $ 0.43万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955113
• 关键词: Antibodies; Antigens; Binding; Carbohydrates; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Enzymes; Funding; Grant; Inositol; Institution; Laboratories; Oxidoreductase; Pathway interactions; Process; Proteins; Research; Research Personnel; Resources; Source; Structure; System; Temperature; Thioredoxin; United States National Institutes of Health; Vitamin K; Work; carboxylate; novel; pressure; protein complex; protein protein interaction; structural biology; thioredoxin reductase

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The work in my laboratory is focused on the structural characterization of novel enzymes and protein complexes. We are currently looking at several different enzymes/systems for these studies. Our work on novel enzymes is being conducted in collaboration with Dr. David Palmer (UofS). We have two projects underway with his group, to determine the structures of *2-Succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC) synthase (MenD)*, and important enzyme in the vitamin K biosynthetic pathway, and *inositol dehydrogenase (IDH)*, a protein that has diverse substrate recognition. Protein-protein interactions are essential for the proper functioning of all cellular systems. In general, there are two types of protein-protein interactions, tight-binding systems such as those seen with antibody:antigen interactions and the formation of protein complexes, and transient or weak binding interactions such as are seen between two proteins interacting in an enzymatic process. The protein-protein interaction system that we are currently studying is the thioredoxin system. Our current research objectives are to probe the interactions between thioredoxin (Trx) and thioredoxin reductase (TrxR) from different species, to characterize the interactions that are important for binding and to examine the way these enzymes are compensating for temperature differences and other evolutionary pressures to maintain their ability to interact with each other.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我实验室的工作重点是新型酶和蛋白质复合物的结构表征。 我们目前正在为这些研究寻找几种不同的酶/系统。 我们正在与 David Palmer 博士（南卡罗来纳大学）合作进行新型酶的研究。我们与他的团队正在进行两个项目，以确定*2-琥珀酰-6-羟基-2,4-环己二烯-1-羧酸酯 (SCHHC) 合酶 (MenD)*（维生素 K 生物合成途径中的重要酶）和*肌醇脱氢酶 (IDH)*（一种具有多种底物识别能力的蛋白质）的结构。 蛋白质-蛋白质相互作用对于所有细胞系统的正常运作至关重要。 一般来说，有两种类型的蛋白质-蛋白质相互作用，紧密结合系统，例如抗体：抗原相互作用和蛋白质复合物的形成，以及瞬时或弱结合相互作用，例如在酶促过程中相互作用的两个蛋白质之间看到的。我们目前正在研究的蛋白质-蛋白质相互作用系统是硫氧还蛋白系统。 我们目前的研究目标是探测不同物种的硫氧还蛋白 (Trx) 和硫氧还蛋白还原酶 (TrxR) 之间的相互作用，表征对结合很重要的相互作用，并检查这些酶补偿温度差异和其他进化压力以保持其相互作用能力的方式。