Investigating the contributions of inflammation and antigen depot to adjuvant function in vivo.

研究炎症和抗原库对体内佐剂功能的贡献。

• 批准号: BB/E00069X/1
• 负责人: James MacDonald Brewer
• 金额: $ 47.18万
• 依托单位: University of Strathclyde
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FE00069X%2F1
• 关键词: Investigating; contributions; inflammation; antigen; depot

While we think that we understand how vaccines work, it is clear that we don't really know how the magnitude and type of immune response a vaccine induces is controlled. This is important, as a number of vaccines are only feasible if we can modulate the size and type of immune response generated. In vaccination, this function is performed by 'adjuvants' and we don't really understand how they work either. Experiments in culture suggest an important role for cells, known as dendritic cells, in controlling vaccine-induced responses. However, cells of the immune system do not work in isolation and it has been difficult to analyse the interactions of dendritic cells and other immune cells in real physiological environments. We have therefore developed a number of state of the art techniques that allow us to characterise the function of dendritic cells, their interactions with other immune cells and the effects of these processes on vaccine-induced responses, in a physiological environment in real time. Only by analysing these interactions in a physiological environment in real time, can we understand how vaccines and adjuvants control the magnitude and type of immune response. This fundamental information will help produce agents to enhance the immune response to vaccines against infections or cancers, as well as to turn off inappropriate immune responses associated with diseases such as allergy, asthma and autoimmunity.

虽然我们认为我们了解疫苗是如何工作的，但很明显，我们并不真正知道疫苗诱导的免疫反应的大小和类型是如何控制的。这一点很重要，因为只有当我们能够调节产生的免疫反应的大小和类型时，许多疫苗才是可行的。在疫苗接种中，这种功能是由“佐剂”执行的，我们也不知道它们是如何工作的。培养实验表明，树突状细胞在控制疫苗诱导的反应中起着重要作用。然而，免疫系统的细胞不能孤立地工作，并且难以分析树突细胞和其他免疫细胞在真实的生理环境中的相互作用。因此，我们开发了许多最先进的技术，使我们能够在真实的时间内在生理环境中观察树突状细胞的功能、它们与其他免疫细胞的相互作用以及这些过程对疫苗诱导的应答的影响。只有通过在生理环境中真实的分析这些相互作用，我们才能理解疫苗和佐剂如何控制免疫反应的大小和类型。这些基本信息将有助于生产试剂，以增强对感染或癌症疫苗的免疫反应，以及关闭与过敏，哮喘和自身免疫等疾病相关的不适当的免疫反应。

项目成果

Antigen depot is not required for alum adjuvanticity.

• DOI: 10.1096/fj.11-184556
• 发表时间: 2012-03
• 期刊: FASEB journal : official publication of the Federation of American Societies for Experimental Biology
• 作者: Hutchison S;Benson RA;Gibson VB;Pollock AH;Garside P;Brewer JM
• 通讯作者: Brewer JM