Day Length Signalling and Crosstalk Between Cytoplasmic and Chloroplastic Calcium Oscillations in Arabidopsis thaliana

拟南芥细胞质和叶绿体钙振荡之间的日长信号传导和串扰

• 批准号: BB/E002692/1
• 负责人: John Love
• 金额: $ 49.57万
• 依托单位: UNIVERSITY OF EXETER
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FE002692%2F1
• 关键词: Day; Length; Signalling; Crosstalk; Between

The production of grain, fruit and vegetables relies on flowering; without flowers, therefore, there would be no agriculture. Plants carefully control the timing of flowering to optimise pollination and resources, and thereby maximise seed production. Flowering occurs at very precise times of the year, but how this is achieved remains a mystery. Since the early 1930's it has been known that plants keep track of the seasons by comparing the ticking of an internal chronometer to the day length they experience at different times of the year. However, we still know very little about how the signals between this internal pacemaker and day length are perceived and understood. This project will research that critical process which allows plants to flower at the best possible time. I will investigate the hypothesis that calcium-signalling pathways are involved in coordinating flowering time. Calcium is an essential signalling molecule in plants. I have previously shown that the internal chronometer generates calcium oscillations that are shaped by the relative duration of days and nights. Work in other laboratories has shown that a sudden calcium increase occurs in the chloroplasts (the organelles where photosynthesis occurs), at nightfall. I propose that co-incidence, or 'crosstalk' between these two calcium signals is involved in floral timing. To investigate this possibility, I will develop new recombinant techniques for manipulating and imaging calcium in living plants. I will combine these technologies with molecular analysis of flowering time genes and classic physiological protocols to elucidate the role of calcium in flowering time. This research will lead to new, important insights into the seasonality of flowering that will have general application to agriculture. For example, the flowering of crops may be extended over a whole season to avoid gluts, reducing waste, storage costs and the need for imports. Controlling flowering time by precision agriculture may therefore result in improved crop management practices and the production of higher quality food that will benefit the farmer, the consumer and the environment.

粮食、水果和蔬菜的生产依赖于开花；因此，没有花就没有农业。植物仔细控制开花时间，以优化授粉和资源，从而最大限度地提高种子产量。花期在一年中非常精确的时间发生，但这是如何实现的仍然是一个谜。自20世纪30年代初以来，人们已经知道，植物通过比较内部计时器的滴答声和它们在一年中不同时间所经历的白天长度来记录季节。然而，我们仍然对这个内部起搏器和白昼长度之间的信号是如何被感知和理解的知之甚少。该项目将研究使植物在最佳时间开花的关键过程。我将研究钙信号通路参与协调开花时间的假设。钙是植物中必不可少的信号分子。我之前已经证明，内部计时器会产生钙振荡，这种振荡是由白天和黑夜的相对持续时间形成的。其他实验室的研究表明，夜幕降临时，叶绿体（光合作用发生的细胞器）中的钙含量会突然增加。我认为，这两种钙信号之间的共同作用或“串扰”与开花时间有关。为了研究这种可能性，我将开发新的重组技术来操纵和成像活植物中的钙。我将把这些技术与开花时间基因的分子分析和经典生理学协议结合起来，阐明钙在开花时间中的作用。这项研究将导致新的，重要的见解开花的季节性，将有普遍应用于农业。例如，作物的开花时间可以延长到整个季节，以避免供过于求，减少浪费、储存成本和进口需求。因此，通过精准农业控制开花时间可能会改善作物管理做法，生产出更高质量的食品，这将使农民、消费者和环境受益。

项目成果

Coccolithophores: functional biodiversity, enzymes and bioprospecting.

• DOI: 10.3390/md9040586
• 发表时间: 2011
• 期刊: Marine drugs
• 影响因子: 5.4
• 作者: Reid EL;Worthy CA;Probert I;Ali ST;Love J;Napier J;Littlechild JA;Somerfield PJ;Allen MJ
• 通讯作者: Allen MJ

An update: improvements in imaging perfluorocarbon-mounted plant leaves with implications for studies of plant pathology, physiology, development and cell biology.

• DOI: 10.3389/fpls.2014.00140
• 发表时间: 2014
• 期刊: Frontiers in plant science
• 影响因子: 5.6
• 作者: Littlejohn GR;Mansfield JC;Christmas JT;Witterick E;Fricker MD;Grant MR;Smirnoff N;Everson RM;Moger J;Love J
• 通讯作者: Love J

How clumpy is my image? Using citizen science to evaluate crowd-sourced annotation tasks.

我的图像有多块状？

• 作者: John Love (Author)