NUTRITIONAL INTAKE AND PROINFLAMMATORY CYTOKINE ACTIVITY IN COMMUNITY DWELLING S

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。在慢性病患者和健康的老年人中，促炎细胞因子水平升高。目前尚不清楚这些细胞因子对健康成年人的营养摄入是否具有与慢性病相同的负面影响。这项拟议的研究的目的是在一组健康的老年人中检查营养摄入和促炎细胞因子活性之间的关系。这项研究将招募40名年龄在65岁或以上、没有慢性病的社区成年人。营养摄入量将使用四天的食物日记进行评估。采血并分析血清中肿瘤坏死因子受体sTNFR1和sTNFR2、IL-1ra、IL-2、IL-4、IL-6、IL-8、IL-10、内毒素、白蛋白、C反应蛋白、尿酸和脂类。24小时的尿液将被收集并分析钠和氮。将使用每人营养缺乏总数的描述性检查来描述该群体的营养摄入量。炎症标志物和营养摄入之间的相互作用将通过比较两组之间的卡路里摄入量、蛋白质摄入量和营养缺乏症的数量来确定，这两组之间的区别是血清细胞因子水平的中位数。膳食脂肪酸摄入量对促炎细胞因子活性的影响将通过比较两组之间的促炎细胞因子水平来确定，根据三种脂肪酸摄入量的中位数进行划分。这项研究将提供数据，以便与我们的心力衰竭患者比较脂肪酸对细胞因子活性的影响，以及细胞因子是否与正常衰老和心力衰竭相关的食物摄入量减少有关。这些信息将有助于根据老年人的健康状况制定适当的干预措施，以改善和维持老年人的营养摄入量。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Proinflammatory cytokines are elevated in people with chronic illness and in healthy older adults. Whether these cytokines have the same negative effect on nutrient intake in healthy adults as in chronic illness is unknown. The purpose of the proposed research is to examine the relationships between nutritional intake and proinflammatory cytokine activity in a group of healthy older adults. Forty community-dwelling adults aged 65 years or older and free of chronic illness will be recruited for the study. Nutritional intake will be assessed using four-day food diaries. Blood will be drawn and serum analyzed for TNF?, soluble TNF receptors sTNFR1 and sTNFR2, IL-1ra, IL-2, IL-4, IL-6, IL-8, IL-10, endotoxin, albumin, CRP, uric acid, and lipid panel. A 24-hour urine will be collected and analyzed for sodium and nitrogen. A descriptive examination of total number of nutrient deficiencies per person will be used to describe the nutritional intake of the group. The interaction among inflammatory markers and nutrtional intake will be determined by comparing calorie intake, protein intake, and number of nutritional deficiencies between two groups divided by a median split of serum cytokine levels. The effect of dietary fatty acid intake on proinflammatory cytokine activity will be determine by comparing proinflammatory cytokine levels between two groups divided by median intake of three fatty acids. This study will provide data for comparison with our heart failure patients with respect to the effects of fatty acids on cytokine activity and whether cytokines are invovled in both the decrease in food intake associated with normal aging and heart failure. This information will assist in developing appropriate interventions to improve and maintain nutritional intake of older adults according to their health status.