Ultrastructure and regulation of adhesion at a genetically tractable model synapse

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Behaviour, learning, memory and cognition are all outward manifestations of the complex neuronal networks that make up the nervous system. Neuronal networks are composed of nerve cells which display long projections forming reproducible points of contact with other nerve, muscle or gland cells. Electrical messages pass along these processes and are transmitted to other cells at the points of contact. Synapses are specialised structures at these contacts which carry out the information transfer. Since they can manipulate or filter information during the transfer process, synapses are key regulators of information flow in neuronal networks, essential for nervous system function and behaviour. Inappropriate function of synapses has dire consequences such as epileptic attacks, mental retardation or death. To execute their function appropriately, a number of structural features have to be correctly established when synapses form during nervous system development. They have to be established at the right places (i.e. at contacts between appropriate cells); synaptic components have to assemble precisely opposite on the signal-sending and -receiving sides of the contact; at each neuronal contact synapses have to assemble in appropriate numbers so that information transfer occurs at a strength adequate for its position in the neuronal network. So called adhesion molecules contribute essentially to all these developmental processes. Adhesion molecules sit on the surface of cells and stick to molecules on other surfaces or of material deposited in the surrounding of cells. Thus, adhesion molecules hold cells together and in certain body locations. The same is true for intercellular adhesion between neurons and their target cells at synapses. Accordingly, a number of synaptic adhesion molecules have been described, but their regulation over time and interdependencies between different classes of adhesion molecules leading to proper synapse development are hardly understood. Here we propose to focus studies of synaptic adhesion on a well known synaptic contact between nerve and muscle cells in a genetic model organism, the fruitfly Drosophila. Drosophila provides numerous advantages speeding up research into genes and their products (such as adhesion molecules). Since genes are often preserved during evolution and are similar between species as distant as humans and the fruitfly, our research will have implications for synapse-related medically relevant research. This project will make extensive use of highly sophisticated imaging techniques (electron microscopy) which allows to view cellular specialisations at extremely high resolution. Here, we will look into the detailed structures formed by adhesion molecules holding neuromuscular contacts of the fruitfly together. From such observations we can deduce, what kind of molecules are involved and whether they differ between different areas of the synaptic contact. In parallel, we will analyse potential defects of synapses in flies with inherited diseases (mutations) affecting adhesion molecules. Together with the structural descriptions, this will help us to pinpoint those molecules required at this particular synaptic contact and determine how they are arranged to carry out their function. Finally, we will try to understand how these molecules function during development in the stepwise process from the early neuromuscular contact to the final appearance and size of the functional synaptic contact.