A new EPR facility for the Centre for Chemical Biology

化学生物学中心的新 EPR 设施

• 批准号: BB/E012418/1
• 负责人: Emma Raven
• 金额: $ 28.07万
• 依托单位: University of Leicester
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FE012418%2F1
• 关键词: new; EPR; facility; Centre; Chemical

There are a number of experimental methods that people use to obtain answers to specific biological questions. In most cases, however, one single technique cannot provide a complete answer. Because of this, it is common practice to use more than one experimental technique and then to combine all the information together to build up a detailed picture of what is happening. One very useful techinque that is under-utilised in the UK is electron paramagnetic resonance (EPR). This method is capable of detecting free (unpaired) electrons. This is useful because the unpaired electrons act as 'spies' on the molecule and can provide information on local environment. This means that EPR can be used to report on biological (free) radicals, on (paramagnetic) metal centres in biological systems and on protein dynamics. At Leicester, we do not have access to an EPR facility that is suitable for biological work. This slows our work down because it means that we cannot obtain some of the information that we need as fast as we would like to. The current proposal seeks funding to address this problem.

人们使用许多实验方法来获得特定生物学问题的答案。然而，在大多数情况下，单一技术无法提供完整的答案。因此，通常的做法是使用多种实验技术，然后将所有信息组合在一起，以详细了解正在发生的情况。电子顺磁共振 (EPR) 是一项非常有用但在英国未得到充分利用的技术。该方法能够检测自由（不成对）电子。这很有用，因为不成对的电子充当分子的“间谍”，可以提供有关局部环境的信息。这意味着 EPR 可用于报告生物（自由基）自由基、生物系统中的（顺磁性）金属中心和蛋白质动力学。在莱斯特，我们无法使用适合生物工作的 EPR 设施。这会减慢我们的工作速度，因为这意味着我们无法像我们希望的那样快地获得我们需要的一些信息。当前的提案寻求资金来解决这个问题。

项目成果

Structure and reaction mechanism in the heme dioxygenases.

• DOI: 10.1021/bi101732n
• 发表时间: 2011-04-12
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Efimov I;Basran J;Thackray SJ;Handa S;Mowat CG;Raven EL
• 通讯作者: Raven EL