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EPR Sample-cell-Resonator Design and Construction

EPR 样品池谐振器设计与构造

基本信息

批准号：
7465398
负责人：
JAMES S HYDE
金额：
$ 3.05万
依托单位：
MEDICAL COLLEGE OF WISCONSIN
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-08-07 至 2010-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7465398
关键词：
Appendix Attention Award Biological Biology Biomedical Research Biophysics Caring Cells Chemistry Class Collaborations Complement Computer software Computer-Aided Design Computers Condition Coupled Data Quality Development Device Designs Dimensions Doctor of Philosophy Electromagnetic Fields Electron Spin Resonance Spectroscopy Electroplating Elements Engineering Environment Equation Evaluation Facility Construction Funding Category Feedback Frequencies Funding Goals Grant Health Services Research Institution International Intuition Iris Laboratories Lead Left Legal patent Life Light Liquid substance Literature Magnetism Medical Methods Modality Modeling Molecular Structure Natural graphite Numbers Paper Performance Phase Physics Poland Polishes Polytetrafluoroethylene Range Research Research Project Grants Resources Sampling Series Silver Site Solutions Speed Spin Labels Spin Trapping Structure Support System Techniques Temperature Thinking United States National Institutes of Health Universities Variant Wisconsin Work Writing aqueous base career cell assembly college design design and construction improved innovation insight membrane model microwave electromagnetic radiation novel parent grant programs research study tissue preparation

项目摘要

DESCRIPTION (provided by applicant): This application is submitted in accordance with PA-02-057, Fogarty International Research Collaboration Award (FIRCA).The research will be done primarily in Poland, at the Jagiellonian University in Krakow in collaboration with Prof. Wojciech Froncisz, PhD, DSc, who is the Foreign Collaborator. It is an extension of NIH grant R01 EB001417, which is the Parent Grant. This proposal is device-design driven. The goal of the proposed work is to improve sensitivity in electron paramagnetic resonance (EPR) spectroscopy of aqueous fluid-phase samples. The proposal is timely because of recent advances in software for finite-element modeling of electromagnetic fields, computers with greatly improved computing speeds, and computer- controlled electric discharge machining (EDM). Computer-aided design will be used for resonators, sample cells and field modulation coils, followed by experimental evaluation. There are three Specific Aims, each of which involves the construction and evaluation in Poland of a sample-containing microwave resonator for EPR spectroscopy: (1) Loop-Gap Resonators (LGR) for Aqueous Samples. An LGR will be developed with ultra-high efficiency. (2) Uniform Field Cavity Resonators for Aqueous Samples. A Q-band uniform field cavity resonator will be developed for use with a novel sample-cell geometry and (3) Aqueous Sample Cell Clusters. A new Multipurpose cavity will be developed for use with layered flat cells. A theme of all three designs is use of EDM graphite, which has recently been proposed by us for EPR resonator construction. The P.I. serves as Director of The National Biomedical EPR Center, P41 EB00198Q, which is a Research Resource. Resonators developed under this FIRCA proposal will enhance the large number of Collaborative and Service Research projects carried out at the Resource. EPR spectroscopy is an important modality in biomedical research. Studies of short-lived radicals detected by spin trapping, of molecular structure using site-directed spin labeling, of biological or model membranes using spin probes, and of cell or tissue preparations are usually carried out in an aqueous environment. Work proposed here will improve the quality of data obtained in these experiments.

描述（由申请人提供）：本申请是根据PA-02-057，Fogarty国际研究合作奖（FIRCA）提交的。研究将主要在波兰克拉科夫的Jagiellonian大学与外国合作者Wojciech Froncisz教授（博士，DSc）合作完成。它是NIH补助金R 01 EB 001417的延伸，这是父母补助金。该提案是器械设计驱动的。所提出的工作的目标是提高灵敏度的电子顺磁共振（EPR）光谱的水相样品。该建议是及时的，因为最近的进步，在软件的有限元模拟电磁场，计算机大大提高了计算速度，和计算机控制的电火花加工（EDM）。计算机辅助设计将用于谐振器，样品池和场调制线圈，然后进行实验评估。有三个具体目标，其中每一个都涉及在波兰建造和评估用于EPR光谱的含样品微波谐振器：（1）用于含水样品的环隙谐振器（LGR）。将以超高效率开发LGR。(2)用于水溶液样品的均匀场腔谐振器。一个Q波段均匀场谐振腔将被开发用于一个新的样品池的几何形状和（3）含水样品池集群。一种新的多用途腔体将被开发用于分层平板电池。所有三个设计的主题是使用EDM石墨，这是最近提出的EPR谐振器的建设。私家侦探担任国家生物医学EPR中心主任，P41 EB 00198 Q，这是一个研究资源。根据FIRCA提案开发的谐振器将加强在资源中进行的大量合作和服务研究项目。电子顺磁共振波谱是生物医学研究中的一种重要手段。通过自旋捕获检测的短寿命自由基的研究，使用定点自旋标记的分子结构的研究，使用自旋探针的生物或模型膜的研究，以及细胞或组织制备的研究通常在水性环境中进行。这里提出的工作将提高在这些实验中获得的数据的质量。