EPR Sample-cell-Resonator Design and Construction

申请者描述(申请人提供)：本申请是根据PA-02-057，Fogarty国际研究合作奖(FIRCA)提交的。研究将主要在波兰克拉科夫的雅盖隆大学与外国合作者Wojciech Froncisz博士、DSC教授合作完成。它是NIH补助金R01 EB001417的延伸，后者是父母补助金。这项提议是以设备设计为导向的。这项拟议工作的目标是提高水相流体样品的电子顺磁共振(EPR)光谱的灵敏度。这项提议是及时的，因为最近在电磁场有限元建模软件、计算速度大大提高的计算机以及计算机控制的电火花加工(EDM)方面取得了进展。谐振器、样品池和场调制线圈将使用计算机辅助设计，随后将进行实验评估。有三个具体目标，每个目标都涉及在波兰建造和评估用于电子顺磁共振波谱的含样品微波谐振器：(1)用于水相样品的环隙谐振器(LGR)。将以超高效率开发一种LGR。(2)用于水相样品的均匀场腔谐振器。将开发一种Q波段均匀场腔谐振器，用于一种新的样品池几何结构和(3)水样品池团簇。将开发一种新的多用途腔体，用于层状平板电池。这三种设计的一个主题都是使用电火花加工石墨，这是我们最近提出的用于EPR谐振器构造的材料。P.I.担任国家生物医学EPR中心主任，P41 EB00198Q，这是一个研究资源。根据这项FIRCA提案开发的共振器将加强在资源中心开展的大量合作和服务研究项目。电子顺磁共振波谱是生物医学研究中的一种重要手段。通过自旋捕获检测到的短寿命自由基的研究，使用定点自旋标记的分子结构的研究，使用自旋探针的生物膜或模型膜的研究，以及细胞或组织制剂的研究通常在水环境中进行。这里提出的工作将提高在这些实验中获得的数据的质量。

DESCRIPTION (provided by applicant): This application is submitted in accordance with PA-02-057, Fogarty International Research Collaboration Award (FIRCA).The research will be done primarily in Poland, at the Jagiellonian University in Krakow in collaboration with Prof. Wojciech Froncisz, PhD, DSc, who is the Foreign Collaborator. It is an extension of NIH grant R01 EB001417, which is the Parent Grant. This proposal is device-design driven. The goal of the proposed work is to improve sensitivity in electron paramagnetic resonance (EPR) spectroscopy of aqueous fluid-phase samples. The proposal is timely because of recent advances in software for finite-element modeling of electromagnetic fields, computers with greatly improved computing speeds, and computer- controlled electric discharge machining (EDM). Computer-aided design will be used for resonators, sample cells and field modulation coils, followed by experimental evaluation. There are three Specific Aims, each of which involves the construction and evaluation in Poland of a sample-containing microwave resonator for EPR spectroscopy: (1) Loop-Gap Resonators (LGR) for Aqueous Samples. An LGR will be developed with ultra-high efficiency. (2) Uniform Field Cavity Resonators for Aqueous Samples. A Q-band uniform field cavity resonator will be developed for use with a novel sample-cell geometry and (3) Aqueous Sample Cell Clusters. A new Multipurpose cavity will be developed for use with layered flat cells. A theme of all three designs is use of EDM graphite, which has recently been proposed by us for EPR resonator construction. The P.I. serves as Director of The National Biomedical EPR Center, P41 EB00198Q, which is a Research Resource. Resonators developed under this FIRCA proposal will enhance the large number of Collaborative and Service Research projects carried out at the Resource. EPR spectroscopy is an important modality in biomedical research. Studies of short-lived radicals detected by spin trapping, of molecular structure using site-directed spin labeling, of biological or model membranes using spin probes, and of cell or tissue preparations are usually carried out in an aqueous environment. Work proposed here will improve the quality of data obtained in these experiments.