EPR Sample-cell-Resonator Design and Construction

描述（由申请人提供）：本申请按照福格蒂国际研究合作奖（FIRCA） PA-02-057提交。这项研究将主要在波兰克拉科夫的雅盖隆大学与外国合作者Wojciech Froncisz教授合作进行。它是NIH拨款R01 EB001417的延伸，即父母拨款。这个建议是由设备设计驱动的。本研究的目的是提高电子顺磁共振（EPR）光谱在水相样品中的灵敏度。由于电磁场有限元建模软件、计算速度大大提高的计算机和计算机控制的电火花加工（EDM）的最新进展，该建议是及时的。计算机辅助设计将用于谐振器、样品单元和场调制线圈，然后进行实验评估。有三个具体目标，每个目标都涉及波兰用于EPR光谱的含样品微波谐振器的构建和评估：(1)用于含水样品的环隙谐振器（LGR）。将开发超高效率的LGR。(2)用于含水样品的均匀场腔谐振器。一个q波段均匀场腔谐振器将被开发用于一个新的样品-细胞几何和(3)水样品细胞团。一种新的多用途腔将被开发用于分层扁平细胞。这三种设计的一个主题是使用EDM石墨，这是我们最近提出的EPR谐振器结构。P.I.担任国家生物医学EPR中心主任，P41 EB00198Q，这是一个研究资源。根据FIRCA提案开发的谐振器将增强在资源中进行的大量协作和服务研究项目。EPR光谱是生物医学研究中的一种重要方法。用自旋捕获法检测短寿命自由基，用定点自旋标记法研究分子结构，用自旋探针研究生物膜或模型膜，以及在水环境中研究细胞或组织制备。这里提出的工作将提高在这些实验中获得的数据的质量。

DESCRIPTION (provided by applicant): This application is submitted in accordance with PA-02-057, Fogarty International Research Collaboration Award (FIRCA).The research will be done primarily in Poland, at the Jagiellonian University in Krakow in collaboration with Prof. Wojciech Froncisz, PhD, DSc, who is the Foreign Collaborator. It is an extension of NIH grant R01 EB001417, which is the Parent Grant. This proposal is device-design driven. The goal of the proposed work is to improve sensitivity in electron paramagnetic resonance (EPR) spectroscopy of aqueous fluid-phase samples. The proposal is timely because of recent advances in software for finite-element modeling of electromagnetic fields, computers with greatly improved computing speeds, and computer- controlled electric discharge machining (EDM). Computer-aided design will be used for resonators, sample cells and field modulation coils, followed by experimental evaluation. There are three Specific Aims, each of which involves the construction and evaluation in Poland of a sample-containing microwave resonator for EPR spectroscopy: (1) Loop-Gap Resonators (LGR) for Aqueous Samples. An LGR will be developed with ultra-high efficiency. (2) Uniform Field Cavity Resonators for Aqueous Samples. A Q-band uniform field cavity resonator will be developed for use with a novel sample-cell geometry and (3) Aqueous Sample Cell Clusters. A new Multipurpose cavity will be developed for use with layered flat cells. A theme of all three designs is use of EDM graphite, which has recently been proposed by us for EPR resonator construction. The P.I. serves as Director of The National Biomedical EPR Center, P41 EB00198Q, which is a Research Resource. Resonators developed under this FIRCA proposal will enhance the large number of Collaborative and Service Research projects carried out at the Resource. EPR spectroscopy is an important modality in biomedical research. Studies of short-lived radicals detected by spin trapping, of molecular structure using site-directed spin labeling, of biological or model membranes using spin probes, and of cell or tissue preparations are usually carried out in an aqueous environment. Work proposed here will improve the quality of data obtained in these experiments.