EPR Sample-cell-Resonator Design and Construction

描述（由申请人提供）：本申请根据 PA-02-057、福格蒂国际研究合作奖 (FIRCA) 提交。该研究将主要在波兰克拉科夫雅盖隆大学与外国合作者 Wojciech Froncisz 教授（博士、理学硕士）合作进行。它是 NIH 拨款 R01 EB001417（即家长拨款）的延伸。该提案是设备设计驱动的。拟议工作的目标是提高水相样品电子顺磁共振（EPR）光谱的灵敏度。该提议是及时的，因为电磁场有限元建模软件、计算速度大大提高的计算机以及计算机控制的放电加工（EDM）方面的最新进展。计算机辅助设计将用于谐振器、样品单元和场调制线圈，然后进行实验评估。有三个具体目标，每个目标都涉及在波兰建造和评估用于 EPR 光谱的包含样品的微波谐振器：(1) 用于水样的环隙谐振器 (LGR)。将开发超高效率的 LGR。 (2) 用于水性样品的均匀场腔谐振器。将开发 Q 波段均匀场腔谐振器，用于新型样品池几何形状和 (3) 水样品池簇。将开发一种新的多用途腔体，用于分层扁平电池。所有三种设计的主题都是 EDM 石墨的使用，我们最近提出将其用于 EPR 谐振器构造。 P.I.担任国家生物医学 EPR 中心 P41 EB00198Q 的主任，该中心是一个研究资源。根据该 FIRCA 提案开发的谐振器将增强该资源开展的大量协作和服务研究项目。 EPR 光谱是生物医学研究的重要方式。通过自旋捕获检测的短寿命自由基的研究、使用定点自旋标记的分子结构的研究、使用自旋探针的生物或模型膜的研究以及细胞或组织制剂的研究通常在水性环境中进行。这里提出的工作将提高这些实验中获得的数据的质量。

DESCRIPTION (provided by applicant): This application is submitted in accordance with PA-02-057, Fogarty International Research Collaboration Award (FIRCA).The research will be done primarily in Poland, at the Jagiellonian University in Krakow in collaboration with Prof. Wojciech Froncisz, PhD, DSc, who is the Foreign Collaborator. It is an extension of NIH grant R01 EB001417, which is the Parent Grant. This proposal is device-design driven. The goal of the proposed work is to improve sensitivity in electron paramagnetic resonance (EPR) spectroscopy of aqueous fluid-phase samples. The proposal is timely because of recent advances in software for finite-element modeling of electromagnetic fields, computers with greatly improved computing speeds, and computer- controlled electric discharge machining (EDM). Computer-aided design will be used for resonators, sample cells and field modulation coils, followed by experimental evaluation. There are three Specific Aims, each of which involves the construction and evaluation in Poland of a sample-containing microwave resonator for EPR spectroscopy: (1) Loop-Gap Resonators (LGR) for Aqueous Samples. An LGR will be developed with ultra-high efficiency. (2) Uniform Field Cavity Resonators for Aqueous Samples. A Q-band uniform field cavity resonator will be developed for use with a novel sample-cell geometry and (3) Aqueous Sample Cell Clusters. A new Multipurpose cavity will be developed for use with layered flat cells. A theme of all three designs is use of EDM graphite, which has recently been proposed by us for EPR resonator construction. The P.I. serves as Director of The National Biomedical EPR Center, P41 EB00198Q, which is a Research Resource. Resonators developed under this FIRCA proposal will enhance the large number of Collaborative and Service Research projects carried out at the Resource. EPR spectroscopy is an important modality in biomedical research. Studies of short-lived radicals detected by spin trapping, of molecular structure using site-directed spin labeling, of biological or model membranes using spin probes, and of cell or tissue preparations are usually carried out in an aqueous environment. Work proposed here will improve the quality of data obtained in these experiments.