Transmission Electron Microscope

这是一个购买最先进的透射电子显微镜（TEM）的建议。TEM是生物学研究的基本技术，因为它是目前在分子水平上非常详细/有利情况下可视化细胞和亚细胞结构的最佳方法。由于这些机器维护起来既昂贵又耗时，我们建议在诺维奇相邻的3个合作机构之间共享显微镜：John Innes中心，食品研究所和东安格利亚大学生物科学系。所提出的显微镜将具有进行亚细胞结构的3D重建的能力，这将克服生物电子显微镜的最重要的限制之一，即通常只产生生物结构的2D图像。该设备将用于培训许多学生和其他科学家使用TEM，这很重要，因为越来越少的生物学家正在学习这些技能。它将为合作机构的广泛研究提供重要组成部分，从John Innes的基础植物细胞生物学和微生物学到IFR的食品科学和东安格利亚大学的生物医学研究。这台新机器将取代三个地点的旧机器，这些旧机器的使用寿命已经结束，不久将无法维修。

This is a proposal to purchase a state-of-the-art transmission electron microscope (TEM). TEM is an essential technique for biological research, because it is currently the best way of visualising cellular and sub-cellular structures in great detail / in favourable cases at the molecular level. Because these machines are expensive and time-consuming to maintain, we propose to share the microscope between 3 partner institutions which are adjacent to each other in Norwich: the John Innes Centre, the Institute of Food Research and the Biological Sciences Department of the University of East Anglia. The proposed microscope would have the capability of carrying out 3D reconstructions of sub-cellular structures, which would overcome one of the most significant limitations of biological electron microscopy / that only a 2D picture of the biological structures is nomally produced. The equipment will be used to train many students and other scientists in the use of TEM, which is important as fewer and fewer biologists are learning these skills. It will provide a crucial part of a wide range of research across the partner institutions, from basic plant cell biology and microbiology at John Innes to food science at IFR and biomedical research at the University of East Anglia. The new machine will replace very old machines at the three sites which have reached the end of their useful life and will soon be impossible to maintain.