RISK OF LOW VIT D STATUS & FUNCTION: RACE, POPULATION ANCESTRY & SKIN REFLECTION

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定是研究者机构。研究表明，维生素D和维生素D反应基因在预防乳腺癌、结肠癌、前列腺癌和肾癌中发挥着重要作用。有研究表明，维生素D和多态性基因（如维生素D受体）患病率的显著种族差异可能是非洲裔美国人癌症发病率较高的一些实质性种族/民族差异的原因。然而，维生素D反应基因功能的种族/民族差异尚不清楚。此外，使用自我报告的种族来确定种族/民族差异可能与维生素D状态和维生素D反应基因的功能有生物学相关性，也可能没有生物学相关性。我们建议调查的差异，血清维生素D和维生素D的反应性淋巴细胞使用三个独立的措施：自我报告的种族，人口血统的基因祖先信息标记物测量，和色素沉着的皮肤反射率测量。具体目标：1）确定低血清维生素D和维生素D摄入量的患病率（50名非裔美国人和50名白色人）; 2）估计研究志愿者淋巴细胞中维生素D应答基因的活性水平;和3）分析维生素D状态的差异（血清和饮食）和维生素D反应基因的活性水平，人口血统和皮肤反射率，以确定哪种措施最好地预测结果的差异。这项研究的潜在益处包括癌症风险中种族/民族差异的分子研究的进展，以及可能在基于人群的研究中有用的分子表型分析的开发。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Sudies suggest a role of vitamin D and vitamin D-responsive genes in the prevention of cancers of the breast, colon, prostate and kidney. It has been suggested that vitamin D and the significant racial variations in the prevalence of polymorphic genes such as the Vitamin D Receptor may account for some of the substantial racial/ethnic disparities in higher cancer incidence seen among African Americans. However, racial/ethnic differences in the function of vitamin D responsive genes are not known. Furthermore, the use of self-reported race in determining racial/ethnic disparities may or may not be biologically relevant to vitamin D status and the function of vitamin D-responsive genes. We propose to investigate the disparities in serum vitamin D and vitamin D responsiveness in the lymphocytes using three separate measures: self reported race, population ancestry as measured by gene ancestry informative markers, and pigmentation as measured by skin reflectance. Specific Aims: 1) Determine the prevalence of low serum vitamin D and vitamin D intake (50 African American and 50 white); 2) Estimate the activity level of vitamin D-responsive genes in the lymphocytes of study volunteers; and 3) Analyze differences in vitamin D status (serum and dietary) and the activity level of vitamin D responsive genes by self-reported race, population ancestry and skin reflectance in order to determine which measure best predicts outcome disparities. The potential benefits of this research include the advancement of molecular studies of racial/ethnic differences in cancer risk, and the development of a molecular phenotyping assay that might be useful in population-based studies.