SUNLIGHT, NUTRITION, SKIN, AND HUMAN ANCESTRY RELATED TO VITAMIN D EXPOSURE AND

与维生素 D 暴露相关的阳光、营养、皮肤和人类血统

• 批准号: 7951354
• 负责人: Robin Taylor Wilson
• 金额: $ 8.27万
• 依托单位: PENNSYLVANIA STATE UNIV HERSHEY MED CTR
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7951354
• 关键词: African American; Age; Area; Blood; CYP3A4 gene; Caucasians; Caucasoid Race; Chronic; Clinical Research; Computer Retrieval of Information on Scientific Projects Database; DNA; Funding; Genes; Genetic; Genotype; Grant; Human; Incidence; Institution; Lymphocyte; Malignant Neoplasms; Measurement; Medial; Melanins; Mixed Function Oxygenases; Participant; Pilot Projects; Play; Prevalence; Research; Research Personnel; Resources; Response Elements; Role; Serum; Serum Calcium Level; Side; Single Nucleotide Polymorphism; Skin; Source; Spottings; Students; Sun Exposure; Sunlight; Time; United States National Institutes of Health; Upper arm; Variant; Vitamin D; Vitamin D3 Receptor; cancer health disparity; indexing; nutrition; sex

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cancer health disparities among African Americans are increasing. Vitamin D is suspected to play a role in several cancers and other chronic conditions with a higher incidence among African Americans. These studies are aimed at understanding if this disparity is due to prevalence of single nucleotide polymorphisms (SNPs) in vitamin D receptor (VDR) or VDR response elements (VDREs). Differences in serum vitamin D between African American and Caucasian participants will be associated with VDR genotype (short/short, short/long, long/long) and polymorphic variants occurring within VDRE located in VDR, CYP24A1 (24-hydroxylase), and CYP3A4. This study will be conducted with 154 healthy African American and 154 healthy Caucasian Penn State student participants during non-Summer months. (A pilot study was conducted previously, thus an additional 104 African American and 104 Caucasian participants are necessary.) Association between the serum vitamin D level and genotypes will be evaluated adjusting for age, sex, genetic ancestry, skin reflectance (melanin index), sunlight exposure and serum calcium levels will be determined. The SNPs will be determined in blood spots and lymphocyte DNA by re-sequencing of areas of the genes and comparing to the reference sequences. A DermaSpectrometer (CyberDerm, Media, Pa., USA) will be used three times on the upper inner side (medial aspect) of each arm with melanin index measurements averaged.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 非裔美国人的癌症健康差异正在增加。 怀疑维生素D在几种癌症和其他慢性病中发挥作用，非洲裔美国人的发生率更高。 这些研究的目的是了解这种差异是否是由于维生素D受体（VDR）或VDR反应元件（VDRES）中单核苷酸多态性（SNP）的流行造成的。非裔美国人和高加索参与者之间血清维生素D的差异将与位于VDR，CYP24A1（24-羟化酶）和CYP3A4的VDR中发生的VDR基因型（短/短，短/短，长/长）和多态性变体有关。这项研究将在非夏季的154名健康非裔美国人和154名健康高加索宾夕法尼亚州立学生参与者中进行。 （先前进行了一项试点研究，因此还需要评估血清维生素D水平和基因型之间的其他104名非裔美国人和104名白种人参与者。 SNP将通过重新测试基因区域并与参考序列进行比较，在血点和淋巴细胞DNA中确定。将使用平均黑色素指数测量值的每个手臂的上侧（内侧），将使用三次的皮肤光谱计（Cyber​​derm，Media，Pa。，USA）。