IDENTIFICATION OF LEUKOCYTE GENES THAT ARE REGULATED BY THYROID HORMONE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。具体目标2涉及识别一组白细胞基因，其表达水平被甲状腺激素改变，与国家儿童医疗中心的埃里克·霍夫曼博士合作。基因表达在两种特定的细胞类型：单核细胞和CD8细胞正在检查。此外，除了微阵列分析外，我们还计划对本研究中甲状腺功能减退和甲状腺功能亢进的受试者进行检测蛋白质组学。14名甲状腺功能低下的受试者已被纳入并完成研究。一名甲状腺功能亢进的受试者被纳入该研究，但不幸的是，在他完成方案之前失去了随访。计划入组20例甲状腺功能减退和20例甲状腺功能亢进，共40例

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Specific aim 2 involves identifying a panel of leukocyte genes whose level of expression is altered by thyroid hormone in collaboration with Dr. Eric Hoffman from Children's National Medical Center. Gene expression in two specific cell types: monocytes and CD8 cells is being examined. In addition, we are also planning to perform detection proteomics, in addition to microarray analyses, in the hypothyroid and hyperthyroid subjects enrolled in this study. 14 hypothyroid subjects have been enrolled in and completed the study. One hyperthyroid subject was enrolled in the subject, but unfortunately was lost to follow-up before he completed the protocol. The planned enrollment is 20 hypothyroid and 20 hyperthyroid subjects for a total of 40 subjects