IDENTIFICATION OF LEUKOCYTE GENES THAT ARE REGULATED BY THYROID HORMONE

受甲状腺激素调节的白细胞基因的鉴定

• 批准号: 7719030
• 负责人: JACQUELINE JONKLAAS
• 金额: $ 1万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7719030
• 关键词: CD8-Positive T-Lymphocytes; Child; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Detection; Enrollment; Funding; Gene Expression; Genes; Grant; Institution; Leukocytes; Medical center; Proteomics; Protocols documentation; Research; Research Personnel; Resources; Source; Thyroid Hormones; United States National Institutes of Health; cell type; follow-up; monocyte

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Specific aim 2 involves identifying a panel of leukocyte genes whose level of expression is altered by thyroid hormone in collaboration with Dr. Eric Hoffman from Children's National Medical Center. Gene expression in two specific cell types: monocytes and CD8 cells is being examined. In addition, we are also planning to perform detection proteomics, in addition to microarray analyses, in the hypothyroid and hyperthyroid subjects enrolled in this study. 14 hypothyroid subjects have been enrolled in and completed the study. One hyperthyroid subject was enrolled in the subject, but unfortunately was lost to follow-up before he completed the protocol. The planned enrollment is 20 hypothyroid and 20 hyperthyroid subjects for a total of 40 subjects

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 具体目标2包括与国家儿童医学中心的埃里克·霍夫曼博士合作，鉴定其表达水平受甲状腺激素影响的一组白细胞基因。目前正在检测两种特定细胞类型：单核细胞和CD8细胞的基因表达。此外，除了微阵列分析外，我们还计划在参与这项研究的甲减和甲亢受试者中进行检测蛋白质组学。14名甲状腺功能减退症受试者参加并完成了这项研究。一名甲亢受试者参加了这项研究，但不幸的是，他在完成方案之前就失去了后续行动。计划招收20名甲状腺功能减退和20名甲亢患者，共40名。