METABOLISM OF GLUTAMINE IN NEWBORN INFANTS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定是研究者机构。适当的营养补充对于早产儿和患病婴儿的管理和护理至关重要。建议在补充配方奶粉中加入谷氨酰胺，因为它可以降低发病率，帮助极低出生体重儿适应宫外环境。本研究旨在检查补充谷氨酰胺对正常健康足月婴儿出生后（24-48小时）或3-4周龄时蛋白质损失和蛋白质周转的代谢影响。 假设：1）谷氨酰胺合成和利用将减少，并且在出生后立即补充期间尿素产生将增加; 2）葡萄糖和氨基酸补充不会影响出生后立即的代谢; 3）支链氨基酸的转氨作用在出生后立即升高; 4）葡萄糖和氨基酸补充增强3-4周龄时的蛋白质增加。 这是一项相对小规模的试验，旨在招募4组（每组N= 15）正常婴儿，采用组间设计。将使用亮氨酸、苯丙氨酸、尿素、谷氨酰胺和碳酸氢钠的稳定同位素输注评估蛋白质和氨基酸周转。此外，还将采集呼吸样本以测量氧化率。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Appropriate nutritional supplementation is essential in the management and care of premature and sick infants. It is recommended that glutamine be included in the supplement formula because it may reduce morbidity and help very low birth weight infants adapt to the extrauterine environment. The present study proposes to examine the metabolic effects of glutamine supplementation on protein loss and protein turnover in normal, healthy term infants in the periods immediately after birth (24-48 hours) or at 3-4 weeks of age. It is hypothesized that: 1) glutamine synthesis and utilization will be reduced and that urea production will be increased during supplementation immediately after birth; 2) glucose and amino acid supplementation will not impact metabolism immediately after birth; 3) transamination of branched amino acids will be high immediately after birth; 4) glucose and amino acid supplementation enhance protein accretion at 3-4 weeks of age. This is a relatively small-scale trial that seeks to enroll 4 groups (N= 15 per group) of normal infants in a between group design. Protein and amino acid turnover will be assessed using stable isotope infusions of leucine, phenylalanine, urea, glutamine and bicarbonate sodium. In addition, breath samples will be obtained to measure oxidation rates.