HUMAN SENSITIVITY TO GENOTOXIC EFFECTS OF BUTADIENE

人类对丁二烯基因毒性作用的敏感性

• 批准号: 7378697
• 负责人: JONATHAN B WARD
• 金额: $ 0.38万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7378697
• 关键词: HUMAN; SENSITIVITY; GENOTOXIC; EFFECTS; BUTADIENE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The study of "Human sensitivity to the genotoxic effects of butadiene" is examining the effects of occupational exposure to 1,3-butadiene, a chemical used to make synthetic rubber and other polymers. It is a known carcinogen in mice and rats and is classified as a probable human carcinogen. We are using measurements called biological markers in a molecular epidemiology study to evaluate the effects of recent occupational exposures. This is done by measuring the frequency of mutations in a reporter gene (hprt) in lymphocytes from workers in rubber and petrochemical plants. Other measures of exposure to butadiene are also made. These mutations may be effects of exposure to butadiene. Workers exposed to about 1ppm of of butadiene (the current allowable exposure limit) were found to have an increased frequency of mutations and increased amounts of a metabolite of butadiene in their urine. Characterization of the kinds and frequencies of specific mutations observed indicated an elevation in deletion mutations in cells from exposed workers. Our obsservations provide an early warning that current levels of occupational exposure to butadiene pose a potential health risk. These results can be used to identify ways in which workers may be further protected from the risk of leukemia.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。“人类对丁二烯遗传毒性效应的敏感性”研究正在审查职业接触1，3-丁二烯的影响，丁二烯是一种用于制造合成橡胶和其他聚合物的化学品。它是一种已知的小鼠和大鼠致癌物，并被归类为可能的人类致癌物。我们在一项分子流行病学研究中使用称为生物标志物的测量方法来评估最近职业暴露的影响。这是通过测量橡胶和石油化工厂工人淋巴细胞中报告基因（hprt）的突变频率来完成的。还制定了其他暴露于丁二烯的措施。这些突变可能是暴露于丁二烯的影响。暴露于约1 ppm丁二烯（目前允许的暴露限值）的工人发现突变频率增加，尿液中丁二烯代谢物的数量增加。观察到的特定突变的种类和频率的特征表明，暴露工人细胞中的缺失突变升高。我们的观察提供了一个早期警告，即目前的丁二烯职业暴露水平构成了潜在的健康风险。这些结果可用于确定进一步保护工人免受白血病风险的方法。