PROTEOMICS STUDIES OF AIRWAY INFLAMMATION

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。哮喘是由肺中的信号蛋白调节的. 假设-哮喘中的信号蛋白可以用新的复杂的实验室技术鉴定。 具体目的和程序（总结）-检查过敏原激发对哮喘和正常受试者肺中蛋白质变化的影响。 实验设计（总结）-进行支气管镜检查，以在吸入激发后从肺部获取组织和液体。将分析组织、体液和血液中蛋白质的差异。 总的目标是确定重要的信号蛋白，调节过敏原诱导的炎症，粘液分泌和哮喘患者气道高反应性。这些研究将验证ICAT（同位素编码的亲和标签）技术的有效性，定量蛋白质组学的后修饰的信号分子。此外，它将开发哮喘气道的信号蛋白质组。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Brief Background - Asthma is regulated by signaling proteins in the lung. Hypothesis - The signaling proteins in asthma can be identified with new sophisticated laboratory techniques. Specific Aims and Procedures (summary) - To examine the effects of allergen challenge on the changes in proteins present in the lungs for asthmatic and normal subjects. Experimental Design (summary) - Perform bronchoscopy to obtain tissue and fluid from the lung after an inhalation challenge. Tissue, fluid, and blood will be analyzed for differences in proteins. The overall goal is to identify important signaling proteins that regulate allergen-induced inflammation, mucus secretion and airway hyperreactivity in asthmatic patients. These studies will validate the usefulness of ICAT (isotope-coded affinity tag) technology for quantitative proteomics of posttranslationally modified signaling molecules. Further it will develop a signaling proteome for asthmatic airways.