PROTEOMICS STUDIES OF AIRWAY INFLAMMATION

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Brief Background - Asthma is regulated by signaling proteins in the lung. Hypothesis - The signaling proteins in asthma can be identified with new sophisticated laboratory techniques. Specific Aims and Procedures (summary) - To examine the effects of allergen challenge on the changes in proteins present in the lungs for asthmatic and normal subjects. Experimental Design (summary) - Perform bronchoscopy to obtain tissue and fluid from the lung after an inhalation challenge. Tissue, fluid, and blood will be analyzed for differences in proteins. The overall goal is to identify important signaling proteins that regulate allergen-induced inflammation, mucus secretion and airway hyperreactivity in asthmatic patients. These studies will validate the usefulness of ICAT (isotope-coded affinity tag) technology for quantitative proteomics of posttranslationally modified signaling molecules. Further it will develop a signaling proteome for asthmatic airways.