AUTOMATED VIDEO MICROFLUOROMETRY AND INJECTION SYSTEM

自动化视频显微荧光测定和注射系统

• 批准号: 3521217
• 负责人: CLAUDE P LECHENE
• 金额: $ 16.1万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-06-13 至 1992-06-12
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521217
• 关键词: biomedical equipment purchase; cells; cellular pathology; fluorescence microscopy; mammalian embryology; microinjections; micromanipulator; physiology; video recording system

This is an application for a shared photometer, quantitative imaging microfluorometry, and microinjection system. The system is composed of an inverted photo microscope with brightfield, phase, DIC and incident fluorescence capability, a photometer, a video intensifier with a CCD camera operated at room temperature, video disc time lapse recorder, an image processor with 4 frames buffer, a programmable automated microscope stage with an automated microinjection system. The system will be linked to an existing computer network and data analysis will benefit from the Prophet Resource. All users have experience in quantitative microfluorescence on living cells or on liquid droplets and a majority of them are experienced in micromanipulation and microinjection. Quantitative microfluorescence, programmed automated stage, time lapse video recording and microinjection will be used to study: pH homeostasis in the early mammalian embryo, Ca2+. H+ and signalling pathways in glomerular cells, glutamine transport in the early mammalian embryo, function of ras p21 protein, ionic balance in hair cells, effect of heparin on mitogen induced proliferation, control of Na/H exchange by extracellular matrix, and fatty acid transport in the intestinal cell. Scheduling, new users, maintenance and future use will be reviewed by a local advisory committee.

这是共享光度计、定量成像的应用程序 显微荧光测定法和显微注射系统。 该系统由一个明视场倒置显微镜、 相位、DIC和入射荧光能力、光度计、视频 增强器与CCD摄像机在室温下操作，视频光盘时间 时间流逝记录仪，图像处理器与4帧缓冲区，可编程 具有自动显微注射系统的自动显微镜载物台。 的 该系统将与现有的计算机网络和数据分析系统相连接 将受益于先知资源。 所有用户都有经验， 活细胞或液滴上的定量显微荧光， 他们中的大多数都有显微操作和显微注射的经验。 定量显微荧光，程序自动化阶段，时间推移 视频记录和显微注射将被用于研究： 早期哺乳动物胚胎，Ca 2+。H+与肾小球内信号通路 细胞，哺乳动物早期胚胎谷氨酰胺转运，ras功能 p21蛋白，毛细胞离子平衡，肝素对丝裂原的影响 诱导增殖，通过细胞外基质控制Na/H交换， 和脂肪酸在肠细胞中的转运。 日程安排新用户 维护和未来使用将由当地咨询委员会进行审查。