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Characterisation of a novel vertebrate mRNA export pathway

一种新型脊椎动物 mRNA 输出途径的表征

基本信息

批准号：
BB/E025293/1
负责人：
Stuart Wilson
金额：
$ 43.08万
依托单位：
University of Sheffield
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2008
资助国家：
英国
起止时间：
2008 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FE025293%2F1
关键词：
Characterisation novel vertebrate mRNA export

项目摘要

A human cell contains a specialised compartment called the nucleus which holds DNA and this is surrounded by the cytoplasm. Sections of the DNA known as genes carry the information to make proteins which are one of the principal building blocks in humans. In the nucleus, copies of genes are made called messenger RNA which then have to be moved from the nucleus through a special hole called the nuclear pore, to the cytoplasm. In the cytoplasm, mRNA is decoded to make proteins. We have discovered a new pathway for moving messenger from the nucleus, through the nuclear pores to the cytoplasm which uses a protein called UIF. In this proposal we will identify other proteins which interact with UIF and investigate how they bind to UIF. We will also investigate how UIF moves around in the cell and how UIF performs its functions in the transport of messenger RNA. These studies will help us understand how genes are turned into proteins in human cells.

人类细胞有一个特殊的隔室，叫做细胞核，它被细胞质包围着，容纳着DNA。被称为基因的DNA片段携带着制造蛋白质的信息，蛋白质是人类的主要组成部分之一。在细胞核中，基因的副本被称为信使RNA，然后必须通过一个叫做核孔的特殊孔从细胞核中移动到细胞质中。在细胞质中，mRNA被解码生成蛋白质。我们发现了一种新的途径，通过核孔将信使从细胞核转移到细胞质，这需要一种叫做UIF的蛋白质。在本提案中，我们将鉴定与UIF相互作用的其他蛋白质并研究它们如何与UIF结合。我们还将研究UIF如何在细胞内移动，以及UIF如何在信使RNA的运输中发挥作用。这些研究将帮助我们了解基因是如何在人类细胞中转化为蛋白质的。

项目成果

期刊论文数量（9）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A.

Burkholderia pseudomallei毒素抑制翻译因子EIF4A的解旋酶活性。

DOI：
10.1126/science.1211915
发表时间：
2011-11-11
期刊：
Science (New York, N.Y.)
影响因子：
0
作者：
Cruz-Migoni A;Hautbergue GM;Artymiuk PJ;Baker PJ;Bokori-Brown M;Chang CT;Dickman MJ;Essex-Lopresti A;Harding SV;Mahadi NM;Marshall LE;Mobbs GW;Mohamed R;Nathan S;Ngugi SA;Ong C;Ooi WF;Partridge LJ;Phillips HL;Raih MF;Ruzheinikov S;Sarkar-Tyson M;Sedelnikova SE;Smither SJ;Tan P;Titball RW;Wilson SA;Rice DW
通讯作者：
Rice DW

TREX exposes the RNA-binding domain of Nxf1 to enable mRNA export.

DOI：
10.1038/ncomms2005
发表时间：
2012
期刊：
Nature communications
影响因子：
16.6
作者：
通讯作者：

Structural basis for the recognition of cellular mRNA export factor REF by herpes viral proteins HSV-1 ICP27 and HVS ORF57.

DOI：
10.1371/journal.ppat.1001244
发表时间：
2011-01-06
期刊：
PLoS pathogens
影响因子：
6.7
作者：
Tunnicliffe RB;Hautbergue GM;Kalra P;Jackson BR;Whitehouse A;Wilson SA;Golovanov AP
通讯作者：
Golovanov AP

UIF, a New mRNA export adaptor that works together with REF/ALY, requires FACT for recruitment to mRNA.

DOI：
10.1016/j.cub.2009.09.041
发表时间：
2009-12-01
期刊：
Current biology : CB
影响因子：
0
作者：
Hautbergue GM;Hung ML;Walsh MJ;Snijders AP;Chang CT;Jones R;Ponting CP;Dickman MJ;Wilson SA
通讯作者：
Wilson SA

An interaction between KSHV ORF57 and UIF provides mRNA-adaptor redundancy in herpesvirus intronless mRNA export.

DOI：
10.1371/journal.ppat.1002138
发表时间：
2011-07
期刊：
PLoS pathogens
影响因子：
6.7
作者：
Jackson BR;Boyne JR;Noerenberg M;Taylor A;Hautbergue GM;Walsh MJ;Wheat R;Blackbourn DJ;Wilson SA;Whitehouse A
通讯作者：
Whitehouse A