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The assembly and function of the TREX complex

TREX复合体的组装和功能

基本信息

批准号：
BB/J015067/1
负责人：
Stuart Wilson
金额：
$ 54.33万
依托单位：
University of Sheffield
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2012
资助国家：
英国
起止时间：
2012 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FJ015067%2F1
关键词：
assembly function TREX complex

项目摘要

In order for the cells of an organism to live they need to synthesise proteins. Within cells a special template called messenger RNA acts as a blueprint for making proteins. mRNA is produced in a special structure inside the cell called the nucleus, which is encapsulated by a membrane with special holes in it called nuclear pores. The messenger RNA is transported from the nucleus, through the nuclear pores to the cytoplasm of a cell which surrounds the nucleus. In the cytoplasm the messenger RNA is decoded to make proteins and this process is essential for cell survival. A special complex of proteins called TREX determines when a messenger RNA is fully processed and ready to leave the nucleus and then helps transport it to the cytoplasm. The TREX complex has many subunits, some of which we have only just discovered. We do not understand what all the subunits of TREX do nor how they all come together to make the complex and transport the messenger RNA. In this proposal we aim to establish how the TREX complex assembles and examine what each subunit within the complex does. Recent work from other scientists has shown that the TREX complex may play other important functions in the cell such as producing special small RNA molecules called short interfering RNAs which interfere with the synthesis of proteins. Therefore our studies on how the TREX complex assembles and works is likely to help us considerably advance our understanding of how proteins are synthesised in cells. Since the TREX complex is conserved from yeast through to man, our studies are likely to have a broad impact in many areas which affect society including food production and understanding what keeps humans healthy.

为了使生物体的细胞存活，它们需要合成蛋白质。在细胞内，一种称为信使RNA的特殊模板充当制造蛋白质的蓝图。mRNA在细胞内的一种特殊结构中产生，称为细胞核，细胞核被一层膜包裹，膜上有一个特殊的孔，称为核孔。信使RNA从细胞核通过核孔运输到细胞质中，细胞质包围着细胞核。在细胞质中，信使RNA被解码以产生蛋白质，这个过程对于细胞生存至关重要。一种称为TREX的特殊蛋白质复合物决定信使RNA何时被完全加工并准备离开细胞核，然后帮助将其转运到细胞质。TREX复合物有许多亚基，其中一些我们刚刚发现。我们不知道TREX的所有亚基是做什么的，也不知道它们是如何聚集在一起形成复合物并运输信使RNA的。在这项提案中，我们的目标是建立TREX复合物如何组装，并检查复合物中的每个亚基做什么。其他科学家最近的研究表明，TREX复合物可能在细胞中发挥其他重要功能，例如产生称为短干扰RNA的特殊小RNA分子，干扰蛋白质的合成。因此，我们对TREX复合物如何组装和工作的研究可能有助于我们大大推进我们对蛋白质如何在细胞中合成的理解。由于TREX复合物从酵母到人类都是保守的，我们的研究可能会在影响社会的许多领域产生广泛的影响，包括食品生产和了解什么使人类健康。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Sequestration of multiple RNA recognition motif-containing proteins by C9orf72 repeat expansions.

DOI：
10.1093/brain/awu120
发表时间：
2014-07
期刊：
Brain : a journal of neurology
影响因子：
0
作者：
Cooper-Knock J;Walsh MJ;Higginbottom A;Robin Highley J;Dickman MJ;Edbauer D;Ince PG;Wharton SB;Wilson SA;Kirby J;Hautbergue GM;Shaw PJ
通讯作者：
Shaw PJ

Mapping interactions between mRNA export factors in living cells.

DOI：
10.1371/journal.pone.0067676
发表时间：
2013
期刊：
PloS one
影响因子：
3.7
作者：
Teng IF;Wilson SA
通讯作者：
Wilson SA

Competitive and cooperative interactions mediate RNA transfer from herpesvirus saimiri ORF57 to the mammalian export adaptor ALYREF.

DOI：
10.1371/journal.ppat.1003907
发表时间：
2014-02
期刊：
PLoS pathogens
影响因子：
6.7
作者：
Tunnicliffe RB;Hautbergue GM;Wilson SA;Kalra P;Golovanov AP
通讯作者：
Golovanov AP

Luzp4 defines a new mRNA export pathway in cancer cells.

DOI：
10.1093/nar/gkv070
发表时间：
2015-02-27
期刊：
Nucleic acids research
影响因子：
14.9
作者：
Viphakone N;Cumberbatch MG;Livingstone MJ;Heath PR;Dickman MJ;Catto JW;Wilson SA
通讯作者：
Wilson SA