COBRE: USC: MOUSE CORE, COLON CANCER

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。老鼠研究核心设施继续为结肠癌研究中心(CCCR)的研究人员提供动物研究支持。Mouse Core支付目标研究人员使用的所有小鼠群体维护所产生的所有笼养费用，为小鼠提供所有用于聚合酶链式反应基因分型的设备和试剂，并为各种研究提供组织采购，以及动物程序方面的技术支持和专业知识。在过去的一年里，我们增加了以下设备和能力：BTX 830电子方形电穿孔系统，与异氟醚小动物麻醉机一起，为小动物手术和体内肌肉内电穿孔提供完全集成的系统；Baker SG303 SterilGARD III层流罩和NAPCO组织培养孵化器，以满足Cobre调查人员的需求。我们与CCCR病理核心合作，收集了ApcMin/+小鼠和PER2 ApcMin/+小鼠的组织，对这些小鼠的组织进行了病理学调查。这一合作成果发表在《国际实验病理学杂志》上。我们还与组织学和成像核心合作，检查了造血干细胞(HSCs)在ApcMin/+的小肠和结肠中的植入情况，并检查了耐药HSCs移植对肿瘤对胸苷合成酶抑制剂的影响。一份手稿正在准备提交给《癌症研究》杂志。一些新的研究人员，包括洛恩·霍夫塞斯(药理学)、丹·迪克森(生物学和南卡罗来纳州癌症中心)和特洛伊·博迪诺(医学院)，已经增加了对老鼠的使用。除了帮助这些研究人员培训他们的人员和购买供他们使用的老鼠品系外，老鼠核心还为这些调查人员提供笼子费用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Mouse Research Core Facility continues to provide animal research support to the investigators of the Center for Colon Cancer Research (CCCR). The Mouse Core pays for all the cage charges incurred in maintaining all mouse colonies utilized by the target investigators, provide all equipment and reagents for PCR genotyping of mice and procurement of tissues for various studies, as well as technical support and expertise in animal procedures. In the past year, we have added the following equipment and capabilities: a BTX 830 ElectroSquare Electroporation System, which together with the isoflurane small animal anesthesia machine, provides a fully integrated system for small animal surgeries and in vivo intramuscular electroporation; and a Baker SG303 SterilGARD III laminar flow hood and a NAPCO tissue culture incubator to meet the needs of COBRE inestigators. We collaborated with the CCCR Pathology Core to collect tissues from the ApcMin/+ mouse and the Per2 ApcMin/+ mouse to carry out a pathological survey on tissues from these mice. This collaboration has resulted in a publication in the International Journal of Experimental Pathology. We also collaborated with the Histology and Imaging Core to examine the engraftment of hematopoietic stem cells (HSCs) in the small intestine and colon of the ApcMin/+ and to examine the effects of transplantation of drug resistant HSCs on tumor response to thymidylate synthase inhibitors. A manuscript is in preparation for submission to the journal Cancer Research. Several new investigators, including Lorne Hofseth (Pharmacology), Dan Dixon (Biology and South Carolina Cancer Center), and Troy Baudino (School of Medicine), have increased their use of mice. The Mouse Core provided the cage costs for these investigators, in addition to assisting them in training their personnel and procuring mouse strains for their use.