MOUSE CORE

鼠标核心

• 批准号: 8360349
• 负责人: MARIA Marjorette PENA
• 金额: $ 19.8万
• 依托单位: UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2013-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360349
• 关键词: Angiogenic Factor; Animal Experiments; Animals; Budgets; Cancer Etiology; Centers of Research Excellence; Cessation of life; Colon Carcinoma; Colorectal; Colorectal Cancer; Complex; Core Facility; Detection; Diagnosis; Equipment; Funding; General Population; Genotype; Goals; Grant; Histology; Housing; Human Resources; Inflammation; Laboratories; Laboratory Animals; Laboratory Research; Location; Maintenance; Mediating; Molecular; Mouse Strains; Mus; National Center for Research Resources; Nitrogen Oxides; Operative Surgical Procedures; PTGS2 gene; Play; Preparation; Principal Investigator; Procedures; Process; Proto-Oncogene Proteins c-myc; Protocols documentation; Public Health; Reagent; Research; Research Infrastructure; Research Personnel; Research Project Grants; Research Support; Resources; Retinoblastoma; Role; Services; Source; South Carolina; Technology; Tissue Procurements; Training; United States; United States National Institutes of Health; Universities; animal resource; anticancer research; base; carcinogenesis; cost; design; mouse model; novel diagnostics; research study; tissue culture; tissue processing

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of the Mouse Core Facility is to provide research support to all projects in the University of South Carolina COBRE-Center for Colon Cancer Research (CCCR) that require the use of mouse models for colon cancer. These include Projects I (T. Baudino), II (L. Hofseth), IV (D. Dixon), and V (J. Thompson) which will use a variety of mouse strains to examine the role of COX2 and c-myc proteins, angiogenic factors, retinoblastoma (Rb) and nitrogen oxide synthase(NOS)-mediated inflammation in colorectal carcinogenesis, and to develop novel diagnostic technologies for the detection of colorectal cancer. The Mouse Core will provide fully equipped research laboratories within the USC Animal Resource Facility (ARF) where investigators of the CCCR can plan and execute complex animal experiments pertinent to their projects at a centralized location in the USC campus. Specifically, the Core will provide support on the following aspects: 1) animal procurement, housing, and colony maintenance; 2) laboratory space, equipment, and reagents; 3) personnel support and expertise on animal handling procedures, designing experiments, tissue procurement and processing, and personnel training; 4) administrative support in developing Animal Use Protocols and grant preparation; 5) additional support from the ARF in training on basic animal handling and animal cage and room cleaning. The Mouse Core will maintain three laboratories for exclusive use by the CCCR investigators and their personnel. These include a Main laboratory for animal experiments, surgeries, and tissue culture; a PCR workstation laboratory to perform all aspects of PCR-based genotyping of the various mouse colonies maintained by the investigators; and 3) a Histology workstation laboratory for tissue procurement, processing, and analysis. The Mouse Core will provide all equipment and reagents for animal experiments, PCR genotyping, and tissue processing from its budget. These services will facilitate the timely execution of animal experiments in the CCCR that are aimed at understanding molecular mechanisms that might play a role in the initiation, progression, diagnosis, and treatment of colorectal cancer. Because colorectal cancer is the second leading cause of cancer deaths in the United States, the results from these research projects will be of great benefit to the general public health.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 小鼠核心设施的目标是为南卡罗来纳大学 COBRE 结肠癌研究中心 (CCCR) 的所有需要​​使用结肠癌小鼠模型的项目提供研究支持。其中包括项目 I (T. Baudino)、II (L. Hofseth)、IV (D. Dixon) 和 V (J. Thompson)，这些项目将使用多种小鼠品系来检查 COX2 和 c-myc 蛋白、血管生成因子、视网膜母细胞瘤 (Rb) 和氮氧化物合酶 (NOS) 介导的炎症在结直肠癌发生中的作用，并开发新的诊断方法 结直肠癌检测技术。 Mouse Core 将在南加州大学动物资源设施 (ARF) 内提供设备齐全的研究实验室，CCCR 的研究人员可以在南加州大学校园的集中地点规划和执行与其项目相关的复杂动物实验。具体来说，核心将在以下几个方面提供支持： 1) 动物采购、饲养和群体维护； 2）实验室空间、设备、试剂； 3) 动物处理程序、设计实验、组织方面的人员支持和专业知识 采购加工、人员培训； 4) 发展动物利用的行政支持 协议和赠款准备； 5) ARF 在基本动物处理以及动物笼子和房间清洁培训方面提供额外支持。 Mouse Core 将维护三个实验室，供 CCCR 调查人员及其人员专用。 其中包括动物实验、手术和组织培养的主要实验室； PCR 工作站实验室，用于对研究人员维护的各个小鼠群体进行基于 PCR 的基因分型的各个方面； 3) 用于组织采购、处理和分析的组织学工作站实验室。 Mouse Core 将从其预算中提供用于动物实验、PCR 基因分型和组织处理的所有设备和试剂。这些服务将有助于 CCCR 及时进行动物实验，旨在了解可能在结直肠癌的发生、进展、诊断和治疗中发挥作用的分子机制。因为 结直肠癌是美国癌症死亡的第二大原因，这些研究项目的结果将对广大公众健康大有裨益。