MOUSE CORE

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 小鼠核心设施的目标是为南卡罗来纳州COBRE-Center for Colon Cancer Research（CCCR）的所有项目提供研究支持，这些项目需要使用小鼠模型进行结肠癌研究。这些项目包括I（T. Baudino），II（L. Hofseth），IV（D.狄克逊）和V（J. Thompson），他们将使用多种小鼠品系来检查COX 2和c-myc蛋白、血管生成因子、视网膜母细胞瘤（Rb）和一氧化氮合酶（NOS）介导的炎症在结肠直肠癌发生中的作用，并开发用于检测结肠直肠癌的新诊断技术。Mouse Core将在USC Animal Resource Facility（ARF）内提供设备齐全的研究实验室，CCCR的研究人员可以在USC校园的集中位置计划和执行与其项目相关的复杂动物实验。具体而言，核心将在以下方面提供支持： 1)动物采购、圈养和群体维护; 2)实验室空间、设备和试剂; 3)动物处理程序、设计实验、组织 采购加工及人员培训; 4)开发动物使用的行政支持 议定书和赠款准备; 5)ARF在基本动物处理和动物笼舍及房间清洁培训方面提供额外支持。 小鼠中心将保留三个实验室，供CCCR研究者及其工作人员专用。 其中包括动物实验、手术和组织培养的主要实验室; PCR工作站实验室，用于对研究者维护的各种小鼠集落进行基于PCR的基因分型的所有方面;以及3）组织学工作站实验室，用于组织采购、处理和分析。Mouse Core将从其预算中提供用于动物实验、PCR基因分型和组织处理的所有设备和试剂。这些服务将促进CCCR及时执行动物实验，旨在了解可能在结直肠癌的发生、进展、诊断和治疗中发挥作用的分子机制。因为 结直肠癌是美国癌症死亡的第二大原因，这些研究项目的结果将对公众健康大有裨益。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of the Mouse Core Facility is to provide research support to all projects in the University of South Carolina COBRE-Center for Colon Cancer Research (CCCR) that require the use of mouse models for colon cancer. These include Projects I (T. Baudino), II (L. Hofseth), IV (D. Dixon), and V (J. Thompson) which will use a variety of mouse strains to examine the role of COX2 and c-myc proteins, angiogenic factors, retinoblastoma (Rb) and nitrogen oxide synthase(NOS)-mediated inflammation in colorectal carcinogenesis, and to develop novel diagnostic technologies for the detection of colorectal cancer. The Mouse Core will provide fully equipped research laboratories within the USC Animal Resource Facility (ARF) where investigators of the CCCR can plan and execute complex animal experiments pertinent to their projects at a centralized location in the USC campus. Specifically, the Core will provide support on the following aspects: 1) animal procurement, housing, and colony maintenance; 2) laboratory space, equipment, and reagents; 3) personnel support and expertise on animal handling procedures, designing experiments, tissue procurement and processing, and personnel training; 4) administrative support in developing Animal Use Protocols and grant preparation; 5) additional support from the ARF in training on basic animal handling and animal cage and room cleaning. The Mouse Core will maintain three laboratories for exclusive use by the CCCR investigators and their personnel. These include a Main laboratory for animal experiments, surgeries, and tissue culture; a PCR workstation laboratory to perform all aspects of PCR-based genotyping of the various mouse colonies maintained by the investigators; and 3) a Histology workstation laboratory for tissue procurement, processing, and analysis. The Mouse Core will provide all equipment and reagents for animal experiments, PCR genotyping, and tissue processing from its budget. These services will facilitate the timely execution of animal experiments in the CCCR that are aimed at understanding molecular mechanisms that might play a role in the initiation, progression, diagnosis, and treatment of colorectal cancer. Because colorectal cancer is the second leading cause of cancer deaths in the United States, the results from these research projects will be of great benefit to the general public health.