MOUSE CORE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 鼠标核心基金的目标是为南卡罗来纳大学科布雷结肠癌研究中心(CCCR)需要使用结肠癌小鼠模型的所有项目提供研究支持。这些项目包括项目I(T.Baudino)、项目II(L.Hofseth)、项目IV(D.Dixon)和项目V(J.Thompson)，这些项目将使用各种小鼠品系来研究COX2和c-myc蛋白、血管生成因子、视网膜母细胞瘤(RB)和一氧化氮合酶(NOS)介导的炎症在结直肠癌发生中的作用，并开发检测结直肠癌的新诊断技术。Mouse Core将在南加州大学动物资源设施(ARF)内提供设备齐全的研究实验室，CCCR的研究人员可以在南加州大学园区的中央位置计划和执行与他们的项目相关的复杂动物实验。具体地说，核心将在以下方面提供支持： 1)动物采购、安置和群体维护； 2)实验室空间、设备和试剂； 3)在动物处理程序、设计实验、组织方面的人员支持和专业知识 采购和加工，以及人员培训； 4)发展畜牧业的行政支持 协议和赠款准备； (5)在基本动物处理和动物笼子和房间清洁方面的培训方面，来自ARF的额外支持。 鼠标核心将维持三个实验室，专供CCCR调查人员及其工作人员使用。其中包括一个用于动物实验、手术和组织培养的主实验室；一个用于对研究人员维护的各种小鼠群体进行基于PCR的基因分型的所有方面的聚合酶链式反应工作站实验室；以及3)用于组织获取、处理和分析的组织学工作站实验室。Mouse Core将从其预算中提供动物实验、聚合酶链式反应基因分型和组织处理的所有设备和试剂。这些服务将有助于及时在CCCR中进行动物实验，旨在了解可能在结直肠癌的发生、发展、诊断和治疗中发挥作用的分子机制。因为 结直肠癌是美国癌症死亡的第二大原因，这些研究项目的结果将对普通公众的健康有很大的好处。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of the Mouse Core Facility is to provide research support to all projects in the University of South Carolina COBRE-Center for Colon Cancer Research (CCCR) that require the use of mouse models for colon cancer. These include Projects I (T. Baudino), II (L. Hofseth), IV (D. Dixon), and V (J. Thompson) which will use a variety of mouse strains to examine the role of COX2 and c-myc proteins, angiogenic factors, retinoblastoma (Rb) and nitrogen oxide synthase(NOS)-mediated inflammation in colorectal carcinogenesis, and to develop novel diagnostic technologies for the detection of colorectal cancer. The Mouse Core will provide fully equipped research laboratories within the USC Animal Resource Facility (ARF) where investigators of the CCCR can plan and execute complex animal experiments pertinent to their projects at a centralized location in the USC campus. Specifically, the Core will provide support on the following aspects: 1) animal procurement, housing, and colony maintenance; 2) laboratory space, equipment, and reagents; 3) personnel support and expertise on animal handling procedures, designing experiments, tissue procurement and processing, and personnel training; 4) administrative support in developing Animal Use Protocols and grant preparation; 5) additional support from the ARF in training on basic animal handling and animal cage and room cleaning. The Mouse Core will maintain three laboratories for exclusive use by the CCCR investigators and their personnel. These include a Main laboratory for animal experiments, surgeries, and tissue culture; a PCR workstation laboratory to perform all aspects of PCR-based genotyping of the various mouse colonies maintained by the investigators; and 3) a Histology workstation laboratory for tissue procurement, processing, and analysis. The Mouse Core will provide all equipment and reagents for animal experiments, PCR genotyping, and tissue processing from its budget. These services will facilitate the timely execution of animal experiments in the CCCR that are aimed at understanding molecular mechanisms that might play a role in the initiation, progression, diagnosis, and treatment of colorectal cancer. Because colorectal cancer is the second leading cause of cancer deaths in the United States, the results from these research projects will be of great benefit to the general public health.