COBRE: RW HOSP: NEW STEM CELL BIOLOGY: CELL SORTER/FLOW CYTOMETRY CORE FACILITY

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。细胞分选器核心将提供骨髓或皮肤干细胞分离和细胞悬液中供体细胞分析的专业知识。核心将由尼古拉·库塔布博士领导。库塔布博士负责RWMC的流量设施，拥有经验丰富的技术人员。此外，马克·杜纳先生是一名经验丰富的干细胞/分选机技术人员，他将把一定比例的时间投入到这一核心工作中。与库塔布博士合作的其他技术人员将能够提供后备和交叉覆盖，尽管他们不是核心的正式组成部分。这一核心采用了来自Cytomation的MoFIO高速细胞分选器。这种分选机能够进行干细胞分离、单细胞沉积和大事件分析。这个核心被安置在一个专门的房间里，在一个已经建立的流式细胞仪设施中。该FACS设施配备了FACS扫描仪和FACS Calibur(BD仪器)。这两台机器都可以作为项目所需的任何分析工作的备份。研究人员将与核心合作，并准备好他们的细胞群体，以便进行分类或分析。核心将常规分离Lin-Rho(Lo)Ho(Lo)、Lin-Hoechst侧细胞和Lin-SCA+CD45-细胞，并分析GFP+或CFDA-SE阳性细胞群体。该核心将为科布雷的所有项目提供服务，为项目1、2、3进行分离和分析工作。核心还将继续致力于开发新的干细胞和加强已知的干细胞分离。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Cell Sorter Core will provide expertise in marrow or skin stem cell separations and analysis of donor cells in cell suspensions. The core will be headed by Dr. Nicola Kouttab. Dr. Koutab runs the flow facility at RWMC and has experienced technicians. In addition, Mr. Mark Dooner is an experienced stem cell/sorter technician and will devote a percentage of his time to this core. The other technicians working with Dr. Koutab will be able to provide backup and cross coverage although they are not a formal component of the Core. This core features a MoFIo highspeed cell sorter from Cytomation. This sorter is capable of carrying out stem cell separations, single cell deposition and large event analysis. This core is housed in a dedicated room within an established flow cytometry facility. This FACS facility is equipped with a FACS scan and a FACS calibur (BD instruments). Both machines can act as a backup for any analysis work required by the projects. Investigators will work with the core and prepare their cell populations for sorting or analysis. The core will routinely separate Lin- Rho(lo)Ho(lo),Lin - Hoechst side populations and Lin - Sca+ CD45- cells and analyze populations for GFP+ or CFDA-SE positive cells. The core will serve all projects in this COBRE carrying out both separations and analysis work for projects 1, 2, 3. The core also remains dedicated to the development of new and the enhancement of already known stem cell separations.