COBRE RW HOSP: NEW STEM CELL BIOLOGY: CELL PHENOTYPING CORE

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This Core will focus on identifying the nature of cells in skin and skeletal muscle after marrow cell transplantation. Donor cells and their specific lineages will be ascertained and colocalized by confocal and deconvolution fluorescent microscopy. Donor cells will be tracked in tissue sections or cell suspensions by expression of GFP, beta-galactosidase or the presence of male DNA. The lineage of identified donor cells will be ascertained by standard morphology, geographical location and immunohistochemistry. Colocalization of donor and lineage markers will be confirmed using confocal or deconvolution fluorescent microscopy. The Core will provide high-level expertise for these approaches. .