COBRE RW HOSP: NEW STEM CELL BIOLOGY: CELL PHENOTYPING CORE

COBRE RW HOSP：新干细胞生物学：细胞表型分析核心

• 批准号: 7382037
• 负责人: Evangelos Van Badiavas
• 金额: $ 7.69万
• 依托单位: ROGER WILLIAMS HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7382037
• 关键词: COBRE; RW; HOSP; NEW; STEM

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This Core will focus on identifying the nature of cells in skin and skeletal muscle after marrow cell transplantation. Donor cells and their specific lineages will be ascertained and colocalized by confocal and deconvolution fluorescent microscopy. Donor cells will be tracked in tissue sections or cell suspensions by expression of GFP, beta-galactosidase or the presence of male DNA. The lineage of identified donor cells will be ascertained by standard morphology, geographical location and immunohistochemistry. Colocalization of donor and lineage markers will be confirmed using confocal or deconvolution fluorescent microscopy. The Core will provide high-level expertise for these approaches. .

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。该核心将重点关注骨髓细胞移植后皮肤和骨骼肌细胞的性质。供体细胞及其特定谱系将通过共聚焦和去卷积荧光显微镜确定和共定位。通过GFP、β-半乳糖苷酶的表达或雄性DNA的存在，在组织切片或细胞悬液中追踪供体细胞。将通过标准形态学、地理位置和免疫组织化学确定已鉴定供体细胞的谱系。将使用共聚焦或去卷积荧光显微镜确认供体和谱系标志物的共定位。核心将为这些方法提供高水平的专门知识。.