COBRE: RW HOSP: P3: RNA INTERFERENCE TO DISSECT STEM CELL POTENTIAL

描述（由申请人提供）： COBRE赠款有4个主要目标：1。建立一个由资深调查人员组成的强大辅导小组; 2.）通过发展核心实验室、行政支持和资源，加强基础设施支持;在罗杰威廉姆斯医疗中心（RWMC）和合作普罗维登斯机构（布朗，米里亚姆医院和罗得岛医院）招聘和保留年轻和成熟的教师，以继续为国家，地区和国家建立一个主要的干细胞研究中心;和4。确定骨髓细胞产生皮肤和肌肉细胞的能力，并确定这些方法在小鼠伤口愈合和肌营养不良模型中的临床潜力，并建立载体系统以使用siRNA抑制干细胞转录途径并评估对干细胞表型的影响，从PU开始。1. 我们已经组建了一个经验丰富的科学家团队，他们将指导3名有前途的年轻研究人员，并准备在不同的研究领域指导其他人。 这项工作是围绕干细胞生物学和可塑性和干细胞的转录调控以及使用siRNA重定向干细胞分化的潜力进行主题协调。 这3个项目及其PI是：项目1 -Evangelos Badiavas博士-研究骨髓细胞转分化（或转化）为皮肤细胞和愈合伤口的能力。 项目2 -Mehrdad Abedi博士-骨髓细胞产生骨骼肌细胞和治疗肌肉疾病的能力。 项目3 -博士. Bharat Ramratnam -干细胞基因调控RNAi. 这些科学项目由行政核心，细胞分选仪/流式细胞术Clore和细胞表型核心支持。 计划概述了继续指导初级P.I. S，和具体的方法来评估P.I. S的进展。并包括一个专门指导P.L.s获得RO 1资金的计划。 机构承诺是强有力的，不依赖于COBRE供资。 还概述了为各机构和COBRE招聘和培养新的初级教员的计划。 COBRE申请的授予将有效地促进干细胞生物学中心的持续发展。 这项资助也有真实的承诺，扩大我们的理解干细胞可塑性和干细胞转录调控和开发临床前模型的伤口愈合和肌肉萎缩症。

DESCRIPTION (provided by applicant): This COBRE grant has 4 major objectives: 1.) The development of a strong mentoring group of established investigators; 2.) The enhancement of infrastructure support through the development of core laboratories, administrative support, and resources; 3.) The recruitment and retention of young and established faculty at Roger Williams Medical Center (RWMC) and collaborating Providence institutions (Brown, Miriam Hospital, and Rhode Island Hospital) in order to continue the establishment of a major stem cell research center for the state, region, and country; and 4.) To define the capacity of marrow cells to produce skin and muscle cells, and to define the clinical potential of these approaches in murine wound healing and muscular dystrophy models, and to establish Vector Systems to use siRNA to inhibit stem cell transcriptional pathways and to assess the effect on the stem cell phenotype, beginning with PU.1. We have assembled an experienced group of scientists who will mentor 3 promising young investigators and are prepared to mentor others in different research areas. The work is thematically coordinated around stem cell biology and plasticity and transcriptional regulation of stem cells and the potential for using siRNA to redirect stem cell differentiation. The 3 projects and their P.I.s are: Project 1 - Dr. Evangelos Badiavas - studies on the capacity of marrow cells to transdifferentiate (or convert) to skin cells and heal wounds. Project 2 - Dr. Mehrdad Abedi - the capacity of marrow cells to produce skeletal muscle cells and treat muscle disorders. Project 3 - Dr. Bharat Ramratnam - Stem cell gene modulation by RNAi. These scientific projects are supported by an Administrative Core, A Cell Sorter/Flow Cytometry Clore, and a Cell Phenotyping Core. Plans are outlined for continued mentoring of junior P.l.s, and specific approaches for evaluating the progress of the P.I.s. and a plan to specifically guide the P.l.s to RO1 funding is included. Institutional commitment is strong and not dependent on COBRE funding. Plans are also outlined for the recruitment and development of new junior faculty to the institutions and COBRE. The award of this COBRE application would effectively facilitate the continued development of The Center for Stem Cell Biology. This grant also has real promise in expanding our understanding of stem cell plasticity and stem cell transcriptional regulation and developing pre-clinical models for wound healing and muscular dystrophy.