CELL SORTER/FLOW CYTOMETRY CORE FACILITY

细胞分选仪/流式细胞术核心设施

• 批准号: 7610570
• 负责人: NICOLA M KOUTTAB
• 金额: $ 29.53万
• 依托单位: ROGER WILLIAMS HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7610570
• 关键词: 5-(6)-carboxyfluorescein diacetate succinimidyl ester; Cell Separation; Cells; Centers of Research Excellence; Computer Retrieval of Information on Scientific Projects Database; Core Facility; Deposition; Development; Event; Flow Cytometry; Funding; Grant; Green Fluorescent Proteins; Housing; Institution; Marrow; PTPRC gene; Population; Research; Research Personnel; Resources; Scanning; Side; Skin; Sorting - Cell Movement; Source; Stem cells; Suspension substance; Suspensions; Time; United States National Institutes of Health; Work; instrument; rho

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Cell Sorter Core will provide expertise in marrow or skin stem cell separations and analysis of donor cells in cell suspensions. Kevin Johnson is the full time stem cell/sorter technician; in addition, Mark Dooner devotes a percentage of his time to this core. This core features a MoFIo highspeed cell sorter from Cytomation. This sorter is capable of carrying out stem cell separations, single cell deposition and large event analysis. This core is housed in a dedicated room. The FACS facility is also equipped with a FACS scan and a FACS calibur (BD instruments). Both machines can act as a backup for any analysis work required by the projects. Investigators will work with the core and prepare their cell populations for sorting or analysis. The core will routinely separate Lin- Rho(lo)Ho(lo), Lin - Hoechst side populations and Lin - Sca+ CD45- cells and analyze populations for GFP+ or CFDA-SE positive cells. The core will serve all projects in this COBRE carrying out both separations and analysis work. The core also remains dedicated to the development of new and the enhancement of already known stem cell separations.

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构为 研究中心，而研究中心不一定是研究者所在的机构。 细胞分选仪核心将提供骨髓或皮肤干细胞分离和细胞悬液中供体细胞分析的专业知识。凯文约翰逊是全职干细胞/分选技术员;此外，马克杜纳致力于他的时间百分比，这一核心。 该核心采用Cytomation的MoFIo高速细胞分选仪。该分选机能够进行干细胞分离、单细胞沉积和大事件分析。这个核心被安置在一个专用的房间里。FACS设施还配备有FACS扫描仪和FACS calibur（BD仪器）。这两台机器都可以作为项目所需的任何分析工作的备份。研究人员将与核心工作，并准备他们的细胞群进行分类或分析。核心将常规分离Lin-Rho（lo）Ho（lo）、Lin-Hoechst侧群和Lin-Sca + CD 45-细胞，并分析GFP+或CFDA-SE阳性细胞群。核心将服务于COBRE中的所有项目，执行分离和分析工作。核心也仍然致力于开发新的和增强已知的干细胞分离。