Neurpeptidergic control of the salivary gland in the black-legged tick, Ixodes sc

黑腿蜱 (Ixodes sc) 唾液腺的神经肽能控制

• 批准号: 7573177
• 负责人: Yoonseong Park
• 金额: $ 22.2万
• 依托单位: KANSAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-12-15 至 2010-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7573177
• 关键词: Aequorin; Alveolus; Amino Acid Sequence; Animals; Antibodies; Arachidonic Acids; Bacteria; Base Sequence; Benign; Bioinformatics; Biological Assay; Biology; Black-legged Tick; Blood; Candidate Disease Gene; Cells; Complement; Complex; Data; Development; Disease; Dopamine; Drosophila genus; Economics; Female; Foundations; Genes; Health; Human; Image; Immune response; Immunohistochemistry; In Situ Hybridization; Industry; Insecta; Investigation; Knowledge; Lead; Ligands; Liquid Chromatography; Location; Longitudinal Studies; Methods; Molecular Weight; Neuropeptides; Osmoregulation; Outcome; Parasites; Pattern; Peptide Receptor; Peptides; Play; Polymerase Chain Reaction; Proteomics; Protozoa; RNA Interference; Reporter; Research; Reverse Transcription; Risk; Role; Salivary; Salivary Glands; Signal Pathway; Signal Transduction; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Surveys; System; Tick Control; Tick-Borne Diseases; Ticks; Time; Vaccines; Virus; Work; base; combinatorial; design; disease transmission; feeding; gene cloning; immunoreactivity; improved; melanophore-dispersing hormone; nerve supply; neuroregulation; pathogen; prevent; public health relevance; receptor; saliva secretion; tandem mass spectrometry; tool

DESCRIPTION (provided by applicant): Ticks are obligatory external parasites that feed on the blood of hosts, and often transmit pathogens, including bacteria, protozoa, and viruses. Tick-borne diseases cause huge economic losses in the animal industry as well as health risks to humans. The salivary secretions of the ticks play an important role in blood feeding by modulating the host response. We propose to investigate the peptidergic system as it relates to control of the salivary gland in the black-legged tick, Ixodes scapularis, which is one of the most important ticks in the northern U.S. as a risk to human heath. Our preliminary study suggests that at least two peptidergic systems are involved in neural control of the salivary gland: putatively, myoinhibitory peptide (MIP) and pigment dispersing hormone (PDF). Specific Aim 1 is to identify candidate neuropeptides relevant to salivary functions. While we already identified candidate neuropeptides in our preliminary study, we will expand the survey by using more comprehensive and combinatorial approaches, proteomics, bioinformatics and immunohistochemistry. Specific Aim 2 will further characterize the MIP which is the neuropeptide strongly supported for the function controlling the salivary glands in the preliminary studies. Cloning of the genes encoding MIP and its receptor will be followed by investigation of their expression patterns and function. This study will provide the first description of neuropeptides controlling the tick salivary gland and will provide a foundation for investigations into the roles of the peptidergic systems in salivary secretion in the next tier of study. The fundamental knowledge obtained from this research is expected to lead to the design of compounds and vaccines that disrupt the tick salivary function. The outcome of the proposed research will have high impacts on the studies in other tick species that have large negative impacts on the economy and health. PUBLIC HEALTH RELEVANCE The outcome of the proposed work will provide an understanding of the basic biology of neural control of the tick salivary gland, which will provide the principles for studying other species of tick, and provide rational approaches to develop environmentally benign acaricidal compounds/strategies. This research is eventually expected to lead to the design of compounds and vaccines to disrupt the salivary functions and to prevent disease transmissions by ticks, ultimately improving human health.

描述（由申请方提供）：蜱是以宿主血液为食的专性外部寄生虫，通常传播病原体，包括细菌、原生动物和病毒。蜱传疾病在畜牧业中造成巨大的经济损失，并对人类造成健康风险。蜱的唾液分泌物通过调节宿主反应在吸血中起重要作用。我们建议调查的肽能系统，因为它涉及到黑腿蜱，肩突硬蜱，这是在美国北方最重要的蜱之一的唾液腺的控制作为一个风险，以人类健康。我们的初步研究表明，至少有两个肽能系统参与唾液腺的神经控制：puplatin，肌抑制肽（MIP）和色素分散激素（PDF）。具体目标1是鉴定与唾液功能相关的候选神经肽。虽然我们已经在初步研究中确定了候选神经肽，但我们将通过使用更全面和组合的方法，蛋白质组学，生物信息学和免疫组织化学来扩大调查。具体目标2将进一步表征MIP，MIP是在初步研究中强烈支持控制唾液腺功能的神经肽。克隆编码MIP及其受体的基因，然后研究其表达模式和功能。这项研究将提供控制蜱唾液腺的神经肽的第一个描述，并将提供一个基础，为调查的肽能系统在唾液分泌中的作用，在下一层的研究。从这项研究中获得的基础知识有望导致破坏蜱唾液功能的化合物和疫苗的设计。拟议研究的结果将对其他蜱虫物种的研究产生重大影响，这些蜱虫物种对经济和健康产生重大负面影响。公共卫生相关性拟议工作的结果将提供蜱唾液腺神经控制的基本生物学的理解，这将为研究其他蜱种提供原则，并提供合理的方法来开发环境友好的杀螨化合物/策略。这项研究最终有望设计出化合物和疫苗来破坏唾液功能，防止蜱传播疾病，最终改善人类健康。