Phylogenetic structural scaling of the appendicular skeleton: relationship with loading regime and locomotor behaviour

附肢骨骼的系统发育结构尺度：与负荷状态和运动行为的关系

• 批准号: BB/F001169/1
• 负责人: Sandra Shefelbine
• 金额: $ 44.7万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2008
• 资助国家: 英国
• 起止时间: 2008 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FF001169%2F1
• 关键词: Phylogenetic; structural; scaling; appendicular; skeleton

Scaling studies examine how structures change in response to increasing animal size. Most previous scaling studies have characterized bone shape with length and midshaft diameter and quantified loading on the bone with the mass of the animal. In this integrated interdisciplinary study we will use engineering analyses, including image analysis, detailed mechanics, kinematics measurements and statistical shape modelling to determine how bones change across a range of species and sizes. We will investigate 5 species from each of the following clades: bipedal birds, mammalian carnivores (cats, dogs, wolves, lions), Macropodoidea (kangaroos, wallabies), bovine artiodactyls (hoofed mammals), and catarrhine primates (terrestrial monkeys). We will use computer tomography images, similar to 3D x-rays, to determine the structural characteristics of the bone that affect the bone's mechanical properties and its ability to withstand load. We will measure the locomotor behaviour of each animal using a multi-camera motion analysis system and force plates. This will allow us to determine joint angles, ground contact forces, limb positions, and stride characteristics that are integral components of locomotor behaviour and hence bone loading regimes. Using a range of statistical techniques we will determine the relationship between bone structure and the locomotor behaviour. The shapes of some irregularly-shaped bones, such as the scapula and pelvis, are difficult to quantify. Unlike long bones where structural measures, such as cross-sectional area, are simple to determine, irregular bones have no obvious dimensions to measure. Statistical shape modelling overcomes this difficulty by creating a virtual model of the bone and comparing its shape to an average bone. The technique considers all variability and highlights were the most variability in shape occurs. These statistical shape models can be used to predict the shape of the corresponding bone in a joint. This technique would prove extremely useful for palaeontologists who often have incomplete skeletons and must predict the shapes of missing bones. It will also show how closely the shapes of articulating bones are linked via their interrelated loading regimes. Bone is made up of tiny spicules called trabeculae. The size and orientation of the trabeculae are influenced by the loading on the bone. Few studies have examined how the trabecular structure varies across species. We will obtain trabecular bone samples from each species and image them using microCT, which has a resolution up to 5 micrometers. We will obtain standard 3D measures of the trabecular structure, such as trabecular thickness and orientation. Trabecular structural measures will be correlated with locomotor parameters in order to determine how the loading, size, and locomotor behaviour influences bone micro-structure. Our study will also take research on musculoskeletal scaling into a new, extremely integrative direction. We have designed a study that (1) examines all bones in the appendicular skeleton at multiple structural levels, (2) uses a wide range of taxa and sizes, (3) applies a mechanistic approach to understanding bone structure and locomotor behaviour, and (4) employs rigorous statistical techniques to determine relationships between structure and function.

缩放研究考察了结构如何随着动物体型的增加而变化。大多数以前的缩放研究已经用长度和中段直径表征了骨形状，并用动物的质量量化了骨上的载荷。在这项综合性的跨学科研究中，我们将使用工程分析，包括图像分析，详细的力学，运动学测量和统计形状建模，以确定骨骼如何在一系列物种和大小中变化。我们将调查来自以下分支的5个物种：双足鸟类，哺乳动物食肉动物（猫，狗，狼，狮子），Macropodoidea（袋鼠，小袋鼠），牛偶蹄动物（有蹄哺乳动物），和catarrhine灵长类动物（陆地猴）。我们将使用计算机断层扫描图像，类似于3D X射线，以确定影响骨骼机械性能及其承受载荷能力的骨骼结构特征。我们将使用多摄像机运动分析系统和测力板测量每只动物的运动行为。这将使我们能够确定关节角度，地面接触力，肢体位置和步幅特征，这些特征是运动行为的组成部分，因此是骨负荷机制的组成部分。使用一系列的统计技术，我们将确定骨骼结构和运动行为之间的关系。一些形状不规则的骨头，如肩胛骨和骨盆，很难量化。与长骨不同，长骨的结构测量（如横截面积）很容易确定，不规则骨没有明显的尺寸可供测量。统计形状建模通过创建骨骼的虚拟模型并将其形状与平均骨骼进行比较来克服这一困难。该技术考虑了所有可变性，并且高光是形状发生的最大可变性。这些统计形状模型可用于预测关节中相应骨骼的形状。这项技术将被证明是非常有用的古生物学家谁往往有不完整的骨骼和必须预测的形状失踪的骨头。它还将显示如何密切的形状连接的关节骨是通过其相互关联的负载制度。骨是由称为骨小梁的微小骨针组成的。骨小梁的大小和方向受骨上负荷的影响。很少有研究探讨了不同物种之间的骨小梁结构是如何变化的。我们将从每个物种中获取骨小梁样本，并使用分辨率高达5微米的microCT对其进行成像。我们将获得小梁结构的标准3D测量，例如小梁厚度和方向。骨小梁结构测量将与运动参数相关，以确定载荷、尺寸和运动行为如何影响骨微结构。我们的研究还将把肌肉骨骼缩放的研究带入一个新的，非常综合的方向。我们设计了一项研究，（1）在多个结构水平上检查无骨骨骼中的所有骨骼，（2）使用广泛的分类群和大小，（3）应用机械方法来理解骨骼结构和运动行为，（4）采用严格的统计技术来确定结构和功能之间的关系。

项目成果

Structure Model Index Does Not Measure Rods and Plates in Trabecular Bone.

• DOI: 10.3389/fendo.2015.00162
• 发表时间: 2015
• 期刊: Frontiers in endocrinology
• 影响因子: 5.2
• 作者: Salmon PL;Ohlsson C;Shefelbine SJ;Doube M
• 通讯作者: Doube M

BoneJ: Free and extensible bone image analysis in ImageJ.

• DOI: 10.1016/j.bone.2010.08.023
• 发表时间: 2010-12
• 期刊: BONE
• 影响因子: 4.1
• 作者: Doube, Michael;Klosowski, Michal M.;Arganda-Carreras, Ignacio;Cordelieres, Fabrice P.;Dougherty, Robert P.;Jackson, Jonathan S.;Schmid, Benjamin;Hutchinson, John R.;Shefelbine, Sandra J.
• 通讯作者: Shefelbine, Sandra J.

3D Morphometric and posture study of felid scapulae using statistical shape modelling.

• DOI: 10.1371/journal.pone.0034619
• 发表时间: 2012
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Zhang KY;Wiktorowicz-Conroy A;Hutchinson JR;Doube M;Klosowski M;Shefelbine SJ;Bull AM
• 通讯作者: Bull AM

Limb bone scaling in hopping macropods and quadrupedal artiodactyls.

• DOI: 10.1098/rsos.180152
• 发表时间: 2018-10
• 期刊: Royal Society open science
• 影响因子: 3.5
• 作者: Doube M;Felder AA;Chua MY;Lodhia K;Kłosowski MM;Hutchinson JR;Shefelbine SJ
• 通讯作者: Shefelbine SJ

Trabecular bone scales allometrically in mammals and birds.

• DOI: 10.1098/rspb.2011.0069
• 发表时间: 2011-10-22
• 期刊: Proceedings. Biological sciences
• 作者: Doube M;Klosowski MM;Wiktorowicz-Conroy AM;Hutchinson JR;Shefelbine SJ
• 通讯作者: Shefelbine SJ