Neural tube development

神经管发育

• 批准号: BB/F002424/2
• 负责人: Jonathan Clarke
• 金额: $ 28.2万
• 依托单位: King's College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2008
• 资助国家: 英国
• 起止时间: 2008 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FF002424%2F2
• 关键词: Neural; tube; development

A key early step during development of the brain and spinal cord is the mechanism of neural tube closure. This is the mechanism that transforms the early brain primordium from a flat sheet of cells into a tube. The tubular structure is then maintained through to adulthood. When this embryonic process goes wrong, neural tube defects such as spina bifida result. We have recently discovered that in the zebrafish embryo this process depends on a novel mode of cell division, which we have termed mirror-symmetric division. We will investigate which molecular mechaisms are important in the regulation of this novel mode of cell division. In particular we will concentrate on a proteins whose role in the development of invertebrate embryos strongly suggest they could contribute to the control of mirror-symmetric divisions. We will also investigate how this division generates cells that are bound together in an arrangement that allows the tubular structure to develop. It is uncertain at present whether other vertebrate embryos employ this specialised mode of division in brain development, but there is some reason to believe that frog embryos and chick embryos could use a similar process. In this work we will determine whether mirror-symmetric divisions are present in the early frog and chick embryo neural tubes. In addition we will determine whether other parts of the embryo that also generate tubular structures (like the gut) also use mirror-symmetric division as a step in the generation of tubes.

大脑和脊髓发育早期的一个关键步骤是神经管闭合的机制。这就是将早期大脑原基从扁平细胞片转变为管状细胞的机制。管状结构一直维持到成年。当这个胚胎过程出错时，神经管缺陷如脊柱裂就会产生。我们最近发现，在斑马鱼胚胎中，这一过程依赖于一种新的细胞分裂模式，我们称之为镜像对称分裂。我们将研究哪些分子机制在这种新的细胞分裂模式的调节中是重要的。我们将特别关注一种蛋白质，它在无脊椎动物胚胎发育中的作用强烈表明，它们可能有助于控制镜像对称分裂。我们还将研究这种分裂是如何产生细胞的，这些细胞以一种允许管状结构发育的排列结合在一起。目前还不确定其他脊椎动物胚胎是否在大脑发育中使用这种特殊的分裂模式，但有一些理由相信青蛙胚胎和鸡胚胎可能使用类似的过程。在这项工作中，我们将确定镜像对称分裂是否存在于早期的青蛙和鸡胚胎神经管。此外，我们将确定胚胎的其他部分是否也产生管状结构（如肠道），也使用镜像对称分裂作为管状结构产生的一个步骤。

项目成果

Mesoderm is required for coordinated cell movements within zebrafish neural plate in vivo.

中胚层是斑马鱼神经板体内协调细胞运动所必需的。

• DOI: 10.1186/1749-8104-9-9
• 发表时间: 2014-04-23
• 期刊: Neural development
• 影响因子: 3.6
• 作者: Araya C;Tawk M;Girdler GC;Costa M;Carmona-Fontaine C;Clarke JD
• 通讯作者: Clarke JD