Time resolved studies of transport in renal epithelial cells

肾上皮细胞转运的时间分辨研究

• 批准号: 7341731
• 负责人: ZONGLU S HUA
• 金额: $ 17.63万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7341731
• 关键词: Affect; Atomic Force Microscopy; Ballistics; Biology; Biophysics; Blood; Calorimetry; Carrier Proteins; Cartoons; Cell Line; Cell Volumes; Cells; Chemicals; Data; Devices; Distal; Doctor of Philosophy; Drug usage; Education; Educational Background; Electron Transport; End Point; Engineering; Epithelial Cells; Epithelium; Fluorescence Microscopy; Goals; Hand; Homeostasis; Hydrostatic Pressure; Intervention; Ions; Kidney; Kidney Diseases; Kidney Part; Knowledge; Laboratories; Liquid substance; Measurement; Measures; Mechanics; Mentors; Metabolism; Methodology; Microfluidic Microchips; Microfluidics; Osmolar Concentration; Pathway interactions; Patients; Pharmacology; Physics; Play; Polycystic Kidney Diseases; Proteins; Regulation; Research; Research Personnel; Resistance; Role; Science; Signal Pathway; System; Technology; Temperature; Time; Training; Tubular formation; Urine; Waste Products; Water; design; experience; extracellular; fluid flow; interest; kidney cell; mathematical model; new technology; reconstruction; sensor; shear stress; solute; trafficking

DESCRIPTION (provided by applicant): My educational background is in physics (B. S. and M.S.) and materials engineering (PhD). My academic research over the last five years has been focused on both ballistic electron transport and microfluidics. I built a microfabricated microfluidic sensor to measure cell volume in real time, and this project caused me to become interested in biomedical sciences. My immediate goal is to train in biology so I may become an independent researcher in kidney biophysics. My long-term goal is to apply my knowledge of physics and materials science to the kidney and hopefully to make a contribution to treating kidney diseases. My current knowledge of biology has come from self-education and is thus rather narrow. I want a more formal training with didactic courses, hands-on laboratory experience and attendance at scientific meetings. I will do research on the regulation of renal cell volume with Dr. Frederick Sachs as mentor. Dr. Sachs is an expert in biophysics of cells, particularly mechanical transduction. Under his guidance I will study the biophysics of how renal cells respond to osmotic and chemical challenges. The research will use the new volume sensor chip. I have five specific goals in this project: 1) optimize the technology of the sensor for minimal fluid exchange time, temperature regulation and stability, 2) measure the dynamics of cell volume regulation in cultured renal epithelial cells; correlate the volume changes with the concentration of intracellular ions using fluorescence microscopy, 3) partition the solute and water flux into separate components using specific pharmacological interventions, 4) to examine the effect of tubular fluid flow and shear stress on epithelial cell volume with particular relevance to polycystic kidney disease, and 5) to build mathematical models of volume regulation to extract physically relevant parameters from the data.

描述（由申请人提供）： 我的教育背景是物理（B）。S.和MS）材料工程（PhD）我在过去五年的学术研究一直集中在弹道电子传输和微流体。我制作了一个微加工的微流体传感器来真实的测量细胞体积，这个项目使我对生物医学产生了兴趣。我的近期目标是接受生物学方面的培训，这样我就可以成为一名肾脏生物物理学方面的独立研究人员。我的长期目标是将我的物理学和材料科学知识应用于肾脏，并希望为治疗肾脏疾病做出贡献。 我目前的生物学知识来自自学，因此相当狭窄。我想接受更正式的培训，包括教学课程、实验室实践经验和参加科学会议。我将与Frederick Sachs博士一起研究肾细胞体积的调节。萨克斯博士是细胞生物物理学方面的专家，特别是机械转导。在他的指导下，我将研究肾细胞如何应对渗透和化学挑战的生物物理学。该研究将使用新的体积传感器芯片。 本课题有五个具体目标：1）优化传感器的技术，使其具有最短的液体交换时间、温度调节和稳定性; 2）测量培养的肾上皮细胞的细胞体积调节动力学;使用荧光显微镜将体积变化与细胞内离子的浓度相关联，3）使用特定的药理学干预将溶质和水通量分成单独的组分，4）检查小管流体流动和剪切应力对上皮细胞体积的影响，其与多囊肾疾病特别相关，以及5）建立体积调节的数学模型以从数据中提取物理相关参数。